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Effects of hydroalcoholic extract of Chenopodium album on growth factors, antioxidant enzymes and expression of some immune-related genes in zebrafish (Danio rerio)
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Pejman Shahriyari1   , Ruhollah Rahimi * , Shafigh Shafiei3 , Farzaneh Nikokhah4 , Hissein Rahimi Pordanjani5 |
1- rrahimi6083@gmail.com
ُShahrekord university, rrahimi@sku.ac.ir , rrahimi@sku.ac.ir
3- sshafiei@sku.ac.ir
4- hosseinrahimi1398@gmail.com
5- hossein.63.rahimi@gmail.com |
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Abstract: (255 Views) |
Abstract:
Introduction: Farmed fish are exposed to several infectious diseases that can reduce the fish yield. Today, the use of medicinal plants as healthy way can decrease the mortality and improve the growth rates to achieve farmed fish sustainability. Chenopodium album contains many bioactive compounds that can be beneficial to the health of organisms. It is evident from the previous studies that this medicinal plant significantly has antimicrobial, antistress, immunostimulants, growth promotion and appetite stimulation properties. Considering the beneficial compounds and properties of the native plant, we aim to investigate the effects of variousthe dose of the hydroalcoholic extract of this plant on growth factors, antioxidant enzymes, and the expression of some immune genes in zebrafish as an important and widely used vertebrate model organism in scientific research.
Materials and methods: C. album was added to a basal diet at the rate of 0, 0.25, 0.50, 1 and 1.5 g kg−1 and Danio rerio was fed this diet for 56 days. A total of 300 D. rerio with an average body weight of 181 ± 1.06 mg were randomly divided into five treatment groups with three replicates. The fish were fed twice a day (at 9:00 AM and 2:00 PM) at the rate of 3% of their biomass. Growth performance was calculated according to their equations and RNA purification and cDNA synthesis were done according to the manufacturer's instruction. Gene expression levels were also assessed applying a Real-Time PCR machine.
Results and Discussion: Final weight and weight gain were not significantly enhanced in treatment groups compared to those of the control (p<0.05). Furthermore, the expression levels of lysozyme, IL10, TNF and TGF genes and the activity levels of antioxidant enzymes CAT and SOD, in treatment groups were significantly higher than the control treatment (p<0.05). This may be due to the existence of polyphenols, phytochemicals, flavonoids, carotenoids and the antioxidant properties of the plant.
Conclusion: According to the results, we saw a better innate immune system in zebrafish following supplementation of a methanolic extract of C. album. Additionally, the zebrafish model seems applicable for elucidation of gene expression on the vertebrate organism and may contribute to our understanding of C. album products in human patients.
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| Keywords: Chenopodium album, growth, gene expression, antioxidant enzymes, zebrafish |
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Full-Text [PDF 475 kb]
(34 Downloads)
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Type of Study: Research |
Subject:
Special
Received: 2024/07/8 | Accepted: 2024/09/1 | Published: 2025/03/30
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