In an experiment to investigate the bio-excitatory effect of ozone gas on the hatching of artemia cysts, it was found that limited blown ozone gas has an effect on significant increasing of the hatching time of artemia cysts, though artemia cycsts are very resistant to ozone. The cysts hatching were done intitialy using 50, 35, 15, 10 and 5 mg of ozone blowing into one-liter containers that lasted for 15, 10, 5, 3 and 1.5 minutes. The hatching rate of artemia for the experimental groups was equal compared to the control group without ozone during 36 hours of hatching (p. >0.05). Analyzes showed that the using ozone reduced the hatching rate, all treatment groups had a significant reduction in hatching (p <0.05) during 24 hours of hatching compared to the control. This reduction in hatching rates were compensated at 36 hours of hatching and became the same among all groups. With less ozonation rate of 2.5, 5 and 10 mg.l^-1 by blowing for 45 seconds, 1 and 3 minutes, respectively, it was observed that among the exprimental containers, 2.5 mg ozone per liter blown at the beginning of hatching, had more significant hatching by about 93.5% compared to the control (85.5%).