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:: Volume 12, Issue 1 (3-2018) ::
JAD 2018, 12(1): 89-98 Back to browse issues page
Effects of dietary administration of hydroalkohalic extract of ferula (Ferula assafoetida) on growth, antioxidant related genes expression in zebrafish (Danio rerio)
Fatemeh Vahedi , Roghareh Safari * , Ali Shabani , Hossein Hoseinifar , Hamed Kolangi Miandareh
Department of Fisheries, Faculty of Fisheries and Environment, Agricultural Sciences and Natural Resources University of Gorgan, Gorgan, Iran,
Abstract:   (5632 Views)

The present study investigates the effect of different levels of Ferula assafoetida hydroalkohalic extract on expression of genes related to antioxidant enzymes (SOD, CAT) and growth (GH, IGF) in Zebrafish (Danio rerio).  For this purpose, Zebrafish with average wight of 0.3+ 0.01 gr fed on diet supplemented with 0%, 0.5%, 1% and 2% of Ferula assafoetida hydroalkohalic extract for 8 weeks. At the end of feeding trial, RNA extracted from liver and brain, cDNA syntesised with Reverse-Transcriptase kit and PCR were done using primers relate to SOD, CAT, GH and IGF, Beta-actin as housekeeping genes. Results showed that expression of genes related to both growth and antioxidant increased in fish fed Ferula compared to control (P<0.05) and the increase was dose dependent.The highest expression were seen in fish fed 2% extract. According to results it seems that using Ferula assafoetida hydroalkohalic extract could have positive effects on Zebrafish growth and antioxidant systems.
 

Keywords: Growth, Antioxidant, Zebrafish, Ferula assafoetida
Full-Text [PDF 578 kb]   (806 Downloads)    
Type of Study: Research | Subject: General
Received: 2017/05/8 | Accepted: 2017/06/5 | Published: 2018/05/25
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Vahedi F, Safari R, Shabani A, Hoseinifar H, Kolangi Miandareh H. Effects of dietary administration of hydroalkohalic extract of ferula (Ferula assafoetida) on growth, antioxidant related genes expression in zebrafish (Danio rerio). JAD 2018; 12 (1) :89-98
URL: http://aqudev.liau.ac.ir/article-1-467-en.html


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Volume 12, Issue 1 (3-2018) Back to browse issues page
نشریه توسعه آبزی پروری Journal of Aquaculture Development
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