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A survey of changes on growth, digestive enzymes, intestine bacterial flora and hematological parameters of rainbow trout (Oncorhynchus mykiss) fed with a nano capsule of Marrubium vulgare and probiotic
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Rahele Mirzaie1   , Farid Firouzbakhsh * , Hosein Oraji1 |
1- Department of Fisheries, Faculty of Animal Sciences and Fisheries, Sari Agricultural Sciences and Natural Resources University, Sari, Iran.
Department of Fisheries, Faculty of Animal Sciences and Fisheries, Sari Agricultural Sciences and Natural Resources University, Sari, Iran., Department of Fisheries, Faculty of Animal Sciences and Fisheries, Sari Agricultural Sciences and Natural Resources University, Sari, Iran. , f.firouzbakhsh@yahoo.com |
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Abstract: (453 Views) |
This study was conducted with the aim of investigating growth changes, digestive enzymes, intestinal bacterial flora and blood parameters of rainbow trout (Oncorhynchus mykiss) fed with nano extract of Marrubium vulgare and probiotic. For this project, rainbow trout with an average weight of 8/66±0.2 g for 8 weeks in 4 treatments (T1: without plant and probiotics, T2: 400 mg plant nano extract and 1g of probiotics, T3: 800 mg of nano plant extract and 1g of probiotics, T4: 1 gram of probiotic with an equal concentration of Lactobacillus plantarum and Bacillus subtilis) per kilogram of diet were tested in 12 tanks with a total of 20. At the end of the period, in addition to the weight of the fish, carcass composition including moisture, protein, fat and ash was measured. Also, in order to measure biochemical factors, blood enzymes and liver enzymes (alkaline phosphatase ALP, alanine aminotransferase ALT and aspartate aminotransferase AST) were taken from the fish at the end of the blood collection period. According to the results of the final weight, protein efficiency and survival percentage in treatment 2 had the highest values compared to other treatments. Also, treatment 3 had the highest food conversion coefficient compared to other treatments, but treatment 2 showed the lowest value of food conversion coefficient among the treatments. Red blood cell, white blood cell, hemoglobin, hematocrit increased significantly by adding 400 mg of nano plant extract and probiotic to the fish diet (p<0.05). The amount of glucose, triglyceride, total protein and the amount of liver enzymes AST, ALT and ALP of the treatments showed a significant difference with each other (p<0.05) and the amount was also lower in the treatments compared to the control group. Amylase enzyme was improved under the effect of probiotic and nano plant extract levels and the highest level was related to treatment 2. Trypsin and chymotrypsin enzymes in treatments 2 and 3 showed a significant difference with the control treatment (p<0.05).
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| Keywords: Oncorhynchus mykiss, Marrubium vulgare, Probiotic, Nano extract. |
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Full-Text [PDF 404 kb]
(148 Downloads)
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Type of Study: Research |
Subject:
Special
Received: 2023/10/8 | Accepted: 2023/12/23 | Published: 2024/11/21
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