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Comparison of the effect of using killed Aeromonas hydrophila bacteria by three methods: heat, formalin and ultraviolet light on survival rate and immune factors in rainbow trout (Oncorhynchus mykiss)
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Taghi Mohammadi Fotami   , Reza Changizi * , Mahdi Hosseinifard , Hosein Khara , Reza Safari |
| Department of Aquaculture, Babol Branch. Islamic Azad University, Babol, Iran |
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Abstract: (1255 Views) |
| Aeromonas hydrophila is one of the most important bacteria in fish farming, which unfortunately has led to an increase in the use of antibiotics in aquaculture. Therefore, in the present study, in order to provide comfort and increase immunity in rainbow trout, we introduced live bacteria and bacteria killed Aeromonas hydrophila by three methods of heat, formalin and UV as intraperitoneal injection with a concentration of 105 to 4 groups of fish with 3 repetitions for each group. According to the results, the bacterial antigen killed by formalin and UV increased white blood cells (P˂0.05) and retained red blood cells. Also, the levels of Igm and complement 3 and 4 in these two groups were better than those in the group exposed to live bacteria and bacteria killed by heat. Total protein was higher in a group of fish exposed to bacteria killed by formalin and UV but albumin levels did not change significantly. Therefore, based on the results; it is possible to expect an increase in fish immunity by using killed Aeromonas hydrophila bacteria by formalin and UV methods. |
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| Keywords: Aeromonas hydrophila, Rainbow Trout, Immunity, Immunoglobulin |
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Full-Text [PDF 518 kb]
(374 Downloads)
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Type of Study: Research |
Subject:
Special
Received: 2020/07/6 | Accepted: 2020/11/1 | Published: 2021/10/2
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