Considering the effect of plant immunostimulants on non-specific immune response in aquatic animals, the present study was conducted to investigate the effects of supplementation of goldfish (Carasius auratus) diet with (Utrica dioica) hydroalcohalic extract on mucosal immune response (total immunoglobin, lysozyme and protease) as well as immune related (TNF-alfa) gene expression. For this purpose 180 fish with an average weight of 0.192 ± 0.014 were randomly divided in 6 aquaria (two replicate) and fed with this extract on three levels: 0, 2 and 4 gr/kg. Evaluation of mucosal immune parameters revealed that supplementation of diet with Achilleamille folium significantly elevated lyzozyme and protease activity in fish fed 4 gr/kg compared to control and 2 gr/kg (P<0.05). Total immunogloubin didn’t show significant difference in fish fed supplemented diet compared to control (P>0.05). Molecular studies on immune gene (TNF-alfa) gene expression revealed significant increase in Achilleamille folium fed diet (P<0.05). Results showed immunomodulatory effects of dietary Utrica dioica extract on skin mocus as well as expression of inflammatory cytokine in gold fish.