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quality indicators of reared rainbow trout sperm in selected Specific Pathogen Free (SPF) farms in the country: First phase
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Mohammad Hassanzadeh Saber *   , Shahrouz Baradaran Noveiri2 , Abolfazl Sepahdari3 , Saltanat Najjar Lashgari4 |
Genetics and Biotechnology Department, International Sturgeon Research Institute (ISRI), Iranian Fisheries Science Institute (IFSRI), Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran., Genetics and Biotechnology Department, International Sturgeon Research Institute (ISRI), Iranian Fisheries Science Institute (IFSRI), Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran. , saber.merag@gmail.com
2- Genetics and Biotechnology Department, International Sturgeon Research Institute (ISRI), Iranian Fisheries Science Institute (IFSRI), Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran.
3- Health and Diseases Department, Iranian Fisheries Science Research Institute (IFSRI), Agriculture Research Education and Extension Organization (AREEO), Tehran, Iran.
4- Genetics and Breeding Department, Tonekabon Cold-Water Fish Research Center, Iranian Fisheries Science Research Institute (IFSRI), Agriculture Research Education and Extension Organization (AREEO), Tehran, Iran. |
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Abstract: (754 Views) |
| In this study, the sperm quality indicators of rainbow trout breeders in selected Specific Pathogen Free (SPF) farms of the country were investigated in order to plan the long-term preservation of rainbow trout sperm through cryopreservation and creating a sperm bank in the next phase. Sperm samples from 24 breeders from six selected SPF farms from West-Azerbaijan and Mazandaran provinces were collected and evaluated. The average density of sperm is 7.83 ± 4 billion per milliliter, the average volume of extracted sperm is 9.2 ± 1.6 ml, the average percentage of motility and its duration were 78.7 ± 10.3% and 26.6 ± 4.4 seconds, the average pH was 7.74 ± 0.23 and the fertilization percentage of the samples was as 92.8 ± 1.2%. The results showed that the pH 7.7-7.8 is the most appropriate value for inducing motility and its increasing or decreasing relative to this value causes a decrease in fertilization efficiency. Also, a positive correlation between pH and sperm motility (r = 0.533) and fertility (r = 0.67) was determined. According to the obtained information, the characteristics of the best sperm sample is selected for long-term storage and the creation of a genetically diverse sperm bank and targeted management of rainbow trout reproduction. |
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| Keywords: Sperm, Oncorhynchus mykiss, Fertilization, SPF. |
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Full-Text [PDF 403 kb]
(206 Downloads)
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Type of Study: Research |
Subject:
Special
Received: 2023/01/16 | Accepted: 2023/04/28 | Published: 2024/06/30
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