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Influence of glucose on biomass growth and pigment production from Spirulina maxima microalga
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Neda Mirhosseini *   , Reza Davarnejad2 , Ahmad Hallajisani3 , Edgar Cano-Europa4 , Omid Tavakoli5 |
Chemical Engineering Department, Arak University, Arak, Iran, Chemical Engineering Department, Arak University, Arak, Iran , n.mir89@yahoo.com
2- Chemical Engineering Department, Arak University, Arak, Iran
3- Caspian Faculty of Engineering, College of Engineering, University of Tehran, Rezvanshahr, Iran
4- Metabolism Laboratory, Department of Physiology, Institute of National Polytechnic (IPN), Mexico City, Mexico
5- College of Chemical Engineering, University of Tehran, Tehran, Iran |
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Abstract: (1554 Views) |
| Nowadays, the uses of natural colors attract the attention of many researchers due to its high applications. Spirulina maxima is a rich source of proteins and pigments. Thus, it is vital to optimize the production of biomass and pigments to choose appropriate concentration and method of adding glucose. In this paper, the impact of alternative and additive glucose in Zarrouk’s medium at various concentrations (0, 0.5, 1 and 1.5 gL-1) on biomass and pigments production like phycobilioproteins, carotenoid and chlorophyll was investigated during the 7 days of cultivation. Microalga was grown in 8 treatments (4 treatment as an additive and 4 treatment as an alternative carbon source) with 3 replications in laboratory temperature and light intensity of 24 ± 2 µmoles/m2/s (24 hours exposure-time). Maximum specific growth rate and doubling time were calculated using nonlinear modeling by Wolfram-Mathematical software. The highest cell concentration (2.39 gL-1) and specific growth rate (0.47 day-1) were obtained from additive glucose source at the highest initial concentration that was 2 times higher than when the glucose was added as an alternative source and 3.3 times higher than the control treatment. As a result, low concentrations of glucose have not caused growth in biomass and photosynthetic pigments production as well as the highest concentration of glucose. In addition, glucose concentration of 1.5 gL-1 could increase the biomass of Spirulina maxima, the content of carotenoid and chlorophyll a, but has no positive effect on the increase of phycobilioproteins content. Compared with glucose as an alternative source, additive glucose with the highest initial concentration of glucose has shown the highest biomass growth, chlorophyll a and carotenoid production. On the other hand, high accumulation of biomass has reduced light penetration in culture medium and phycobilioproteins production during the last cultivation days. Moreover, adding glucose as an additive and alternative carbon source have not a significant effect on the production of phycobilioproteins pigment. |
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| Keywords: Glucose, Alternative, Additive, Biomass, phycobilioproteins, Spirulina Maxima |
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Full-Text [PDF 1124 kb]
(818 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2019/12/3 | Accepted: 2020/06/26 | Published: 2021/03/30
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