This study was carried out with the aim of detecting the effects of carotenoid astaxanthin on immunonological indices and determining caretonoid residue levels in Acipenser ruthenus. 4 doses of astaxanthin including 0 (control), 15 mg/kg, 45 mg/kg and 75 mg/kg were added to fish diet. A total of 48 pre - broodstock of Acipenser ruthenus (with mean weigth 600 ± 14.5 g) were selected and transferred to fiberglass 500 lit tanks and fed with diet containing different levels astaxanthin during 6 momths. After reproduction, the residue levels of astaxanthin were monitored during emboyos gdevelopment. Based on results, the most percentage of fertitlity among different treatments of astaxanthin was observed at concentration 75 mg/kg (P<0.05). Results showed that hatching rate of eggs in control was lower significantly than concentrations 45 and 75 mg/kg (P<0.05). Minimum residual level of total carotenoid and astaxanthin observed at stage PY and showed significant difference compared to other treatments (P<0.05). Minimum residual levels of cantazantine and Beta - carotene observed at stage PY and showed significant difference compared to ovulation stage (P<0.05). Lysozyme levels showed no significant difference compared to control (P>0.05). IgM levels at concentrations 45 and 75 mg/kg was lower significantly compared to 15mg/kg (P<0.05). Results showed C₄ levels at concentration 45 mg/kg was lower significantly compared to other treatments (P<0.05). Based on results, C₃ levels at concentrations 45 and 75 mg/kg was lower significantly compared to other treatments (P<0.05). By notice to obtained results, Adding 75 mg/kg to diet improved immunity efficiency compared to control. So it suggests adding astaxanthin in Acipenser ruthenus diet.