|
Investigation of genetic structure of Caspian kutum (Rutilus frissi) stocks on the southern basin of the Caspian Sea (Guilan province) by mtDNA sequencing
|
Mohammad Hassanzadeh Saber *   , Omid Jafari , Shirin Jamshidi , Mahmoud Mohseni , Keywan Abbasi Ranjbar , Siamak Bagheri , Esmail Abdolahzadeh , Maryam Forouzad |
| 1-International Sturgeon Research Institute (ISRI), Iranian Fisheries Science Institute (IFSRI), Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran , saber.merag@gmail.com |
|
|
Abstract: (146 Views) |
Introduction: In order to investigate the possibility of genetic differentiation and to compare the genetic structure of Kutum population using DNA sequencing methods, ten Kutums from the eastern, middle and western regions of the Caspian Sea (coasts of Gilan province) were sampled from the mentioned areas.
Materials and methods: DNA of three samples of each area and species was extracted using ammonium acetate method, their quantity and quality were determined using 1% agarose gel and Nanodrop device (ND1000), respectively. One pair of primers (Forward and Reverse) of 16SrRNA gene were designed and synthesized using GeneRuner software. In order to determine the sequence of the 16SrRNA gene, Kutum DNA were amplified by PCR. Then, PCR product was runned on 1% agarose gel and bands have been produced in the range of 500-600 bp.
Results and Discussion: Evolutionary difference (Maximum Likelihood), neighbor-joining and phylogeny relationships were obtained using MEGA11 software. The results show that the samples of Kutum in three areas have 97% similarity and 3- 5% evolutionary differences with each other. The phylogeny tree of Kutum samples was drawn using Neighbor-Joining methods. In order to compare of phylogeny relationships of Kutum from three areas using Neighbor-Joining method, each western and eastern samples were placed in a separated cluster, which it could be a sign of population differences according to 16SrRNA gene. Due to the destruction of the rivers where they migrate, the pollution of the Caspian Sea and the lack of physical barriers in it, the kutum in these two regions still has genetic diversity.
Conclusion: So preserving the genetic structure of the kutum populations in the central, eastern and western regions on the coasts of Gilan province is important and essential. |
|
| Keywords: Genetic structure, Kutum, Golden grey mullet, Caspian Sea, mtDNA. |
|
|
|
Type of Study: Research |
Subject:
Special
Received: 2024/06/9 | Accepted: 2024/06/10 | Published: 2025/07/1
|
|
|
|
|
|
|
| Send email to the article author |
|
|
|
| Add your comments about this article |
|
|
|
