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Evaluation of using the hyperosmotic environment on the Yersinia ruckeri vaccine efficacy by bath method in juvenile great sturgeon (Huso huso).
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Hamidreza Tabibi11   , Reza Afshar Moghaddam1 , Abdolsalam Hatami ZharAbad1 , Mahyar Yousefi Siahkalroodi2 , Somayeh Namrudi3 , Mohammad Mazandarani * |
1- Department of Fisheries, Faculty of Fisheries and Environment, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran, mazandarani57@gmail.com
2- Department of Aquatic Health and Diseases, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran
3- Department of Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan Iran |
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Abstract: (27 Views) |
Introduction: In this study, the effectiveness of Yersinia ruckeri vaccine was assessed using both the bath and injection methods on great sturgeon (Huso huso).
Materials and Method: Six experimental groups were formed, including a group of fish vaccinated by injection method (treatment 1), group of fish vaccinated in two stages using the hyperosmotic solution+vaccine by bath method (treatment 2), a group of fish vaccinated in two stages using the vaccine by bath method (treatment 3), a group of fish vaccinated in one stage using the hyperosmotic solution+vaccine (treatment 4), a group of fish vaccinated in one stage by the bath method (treatment 5), and a control group (treatment 6). For each group, 20 fish were used in two replications, totaling 120 fish with an average weight of 24.6±1.5, which were distributed across 12 fiberglass tanks with a water volume of 140 liters. In this experiment, the second stage of vaccination for the two-stage vaccinated fish was administered 21 days after the first stage, and sampling and bacterial exposure were conducted 42 days after the first vaccination.
Results and Discussion: Based on the results the total protein and serum immunoglobulin levels and the serum antibody titer against Yersinia rockeri was higher in fish vaccinated by injection method compared to other treatments (p≥0.05). On the other hand, in fish vaccinated by bath method, there was no significant difference in these parameters compared to the control (p<0.05). The highest number of white blood cells (WBC) was observed in fish vaccinated by injection method, followed by fish vaccinated by two-step bath method (p≥0.05). No significant difference was recorded between the fish that received one-step vaccine by bath method and the control (p˃0.05). After the bacterial challenge, the control group had the highest mortality rate (77.8%), while the lowest mortality rate was recorded in the fish vaccinated by injection method (33%). In the analysis of serum alkaline phosphatase (ALP), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) levels, no significant differences were observed in all the studied groups. Therefore the one-step vaccination by bath method did not provide adequate immunity in great sturgeon.
Conclusion: In conclusion, it seems that vaccination by injection method has good efficacy with no side effects in this fish. |
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| Keywords: Yersinia ruckeri, Huso huso, vaccination, bacterial challenge, antibody titer |
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Full-Text [DOCX 65 kb]
(13 Downloads)
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Type of Study: Research |
Subject:
Special
Received: 2024/05/25 | Accepted: 2025/07/26 | Published: 2025/07/1
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