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Performance Comparison of Optimized Multiplex Polymerase Chain Reaction with Standard Kit (IQ2000) for White Spot Virus Detection in Shrimp
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Hossein Moein1   , Farshid Kafilzadeh * , Mohammad Khalil Pazir3 , Mohammadrez Miri4 , Tahmineh Nowrouzi4 |
1- Department of Microbiology, Jahrom Branch, Islamic Azad University, Jahrom, Iran.
Department of Microbiology, Jahrom Branch, Islamic Azad University, Jahrom, Iran., Department of Microbiology, Jahrom Branch, Islamic Azad University, Jahrom, Iran. , f.kafilzadeh@gmail.com
3- National Shrimp Research Institute, Iranian Fisheries Science Research Institute, Agricultural Research, Education, and Extension Organization (AREEO), Bushehr, Iran
4- Persian Gulf Marine Biotechnology Research Center, Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran. |
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Abstract: (379 Views) |
| One of the main causes of widespread mortality in farmed shrimp is the white spot syndrome virus (WSSV), which can lead to rapid and significant losses within just a few days. Timely diagnosis can prevent extensive financial losses. The only reliable early detection method for this virus is molecular. Primers compatible with each other were designed from the viral genome for the VP28 region and the protein kinase-related region, and from the shrimp genome for the S18 ribosomal region as an internal control. After optimizing the primers for multiplex detection of WSSV, DNA extracted from 200 shrimp samples suspected of being infected with the virus was compared using two optimized kits and the IQ2000 reference kit (from Taiwan). Of these, 94 samples tested positive (infected with the virus) and 106 samples tested negative, with results matching the reference kit except for two samples. The sensitivity and specificity of the designed kit, compared to the reference kit, were 98% and 100%, respectively. Based on these results, the optimized one-step method from this study can be used as a replacement for or in conjunction with the reference kit. |
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| Keywords: White Spot Virus, Multiplex Polymerase Chain Reaction, Detection, Shrimp, Optimization, IQ2000. |
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Full-Text [PDF 325 kb]
(103 Downloads)
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Type of Study: Applicable |
Subject:
Special
Received: 2021/11/20 | Accepted: 2022/01/24 | Published: 2024/09/22
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