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Effect of dietary copper sulfate replacement with copper oxide nanoparticles on digestive enzymes activity and intestinal histopathology in Gold fish (Carassius auratus)
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Soheila Karimian kakolaki   , Fardin Shaloui * , Mohammad Shadakhtah , Mehdi Arab Markadeh |
| Department of Fisheries and Environment, Faculty of Natural Resources and Earth Sciences, Shahrekord University, Shahrekord, Iran |
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Abstract: (845 Views) |
| Copper (Cu) is an essential micronutrient in animals for normal growth and development of bones, connective tissue, the heart and several other organs, since around 30 enzymes are known to use copper as a cofactor. The commercial diet should provide the essential amount of Cu, which depends on the physical and chemical properties of the form of the supplement in which the Cu is given in the diet. However, the feed industry still prefers CuSO4 for economic reasons. Thus this study was conducted to evaluate the effects of different sources of copper (Cu) on digestive enzymes activity and pathological alterations in goldfish, Carassius auratus. The five experimental diets were formulated to contain different levels of Cu-NPs (0, 3, 5 and 10 mg/kg) and 3 mg/kg CuSO4 and given to goldfish (initial average weight and length 7.78 ±0.67 g and 5.86 ± 0.43 cm respectively) for 8 weeks. Intestinal protease and lipase activities increased and amylase decreased by Cu-NPs compared with the control group (P<0.05). In this study, feeding goldfish with CuSO4 led to significant pathological alterations in intestinal tract. The overall result recommends adding 3 mg Cu-NPs per kg diet improve health and intestinal digestive where the same dose of CuSO4 induce intestinal tract negative impacts in goldfish. However, further studies are needed to explain the molecular mechanisms of Cu-NPs as well lower concentrations of CuSO4. |
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| Keywords: Copper Sulfate, Copper Nanoparticles, digestive enzymes, Histology, intestine, Goldfish |
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Full-Text [PDF 822 kb]
(297 Downloads)
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Type of Study: Research |
Subject:
Special
Received: 2020/01/3 | Accepted: 2020/05/1 | Published: 2021/07/1
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