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Investigation of histological effects of 5-(4-methyltiobenzylidene) thiazolotriazol on liver enzyme changes & extracellular structures of Trichogaster trichopteru’s liver
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Mahsa Mazandarani   , Tahereh Naji * , Homayoun Hosseinzadeh , Ali Almasirad |
| Department of Basic sciences, Faculty of Pharmacy & Pharmaceutical Sciences, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran |
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Abstract: (1564 Views) |
| The aim of this study was to investigate the histological effects of 5-(4-methyltiobenzylidene) thiazolotriazol on liver tissue of Trichogaster trichopterus which was done at the fish lab of IAUPS. Therefore 150 fishes with the weight of 2±0.5 gr were prepared from Khan-mahi fish farm in the May 2019. This study was performed on 4 treatments 10, 20, 30, 40 mg/kg.b.w. Before dropping fishes into the aquariums, dechlorination of water was performed in 24 hours & physicochemical factors was measured too. Injections were performed the day after another in 20 days & all of the treatments were anatomized & their livers put into formalin in order to check by optimal microscope later. Liver enzymes consist of ALT, AST & ALP were send to Laboratory. Results showed that ALT level changed from 1707 u/kg in control 1 up to 1846.6 in treatment 40 mg/kg.b.w , AST level changed from 16.6 u/kg in control1 up to 29 u/kg in treatment 40 mg/kg.b.w , ALP level changed from 716.6 u/kg in control 1 up to 888.6 u/kg in treatment 40 mg/kg.b.w and this results showed significant difference between control & treatment(P≤0.05).optical and study showed some ruptures in liver tissue, increasing spaces between sinusoids and it caused to form fat vacuole in the liver by increasing of doses. |
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| Keywords: Histology, Trichogaster trichopterus, thiazolotriazol |
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Full-Text [PDF 1032 kb]
(437 Downloads)
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Type of Study: Research |
Subject:
Special
Received: 2019/12/25 | Accepted: 2020/09/18 | Published: 2021/10/2
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