[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 12, Issue 2 (5-2018) ::
3 2018, 12(2): 1-11 Back to browse issues page
Assessing Different Methods of DNA Extraction from The golden Rainbow trout farmed (Golden Rainbow Trout: Oncorhynchus mykiss) in order to study the molecular
Bagareh Akbarnejad , Siyamak Yousefi Siahkalroodi , Shihreh Zare Karizi
Islamic Azad University_Varamin-Pishva Branch
Abstract:   (202 Views)
DNA extraction is a routine step in many biological studies and variety of methods have been established to isolate DNA molecules from biological materials. most methods share three aims: 1- the comprehensive lysis of cells and extraction of intracellular nucleic acids into aqueous solution, 2-  the removal of non-nucleic acid organic and inorganic molecules from resultant aqueous extracts  and 3- the minimization of nucleic acid losses throughout this purification process. Rainbow trout fish, including salmonid species have been domesticated and today the most important cold-water fish species in the country. To accomplish this study 4 Extraction 1. Extraction Kit Company Gena All 2- Trizol Reagent 3- traditional method of heating and Proteinase K 4- phenol chloroform were studied  and It was found that the The best extraction method in terms of quality and the quantity of DNA extracted by 1 percent agarose gel and Nanodrop and Also in terms of the effectiveness of PCR for DNA extraction kit method is the enterprise Gene All Country South Korea.
Keywords: Golden rainbow trout farmed, DNA extraction, Company extraction kit Gene All, PCR.
Full-Text [PDF 671 kb]   (60 Downloads)    
Type of Study: Research | Subject: Special
Received: 2018/07/15 | Accepted: 2018/07/15 | Published: 2018/07/15
Add your comments about this article
Your username or Email:

CAPTCHA code


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Akbarnejad B, Yousefi Siahkalroodi S, Zare Karizi S. Assessing Different Methods of DNA Extraction from The golden Rainbow trout farmed (Golden Rainbow Trout: Oncorhynchus mykiss) in order to study the molecular. 3. 2018; 12 (2) :1-11
URL: http://aqudev.liau.ac.ir/article-1-566-en.html


Volume 12, Issue 2 (5-2018) Back to browse issues page
نشریه توسعه آبزی پروری Journal of Aquaculture Development
Persian site map - English site map - Created in 0.05 seconds with 31 queries by YEKTAWEB 3768