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Assessing Different Methods of DNA Extraction from The golden Rainbow trout farmed (Golden Rainbow Trout: Oncorhynchus mykiss) in order to study the molecular
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Bagareh Akbarnejad   , Siyamak Yousefi Siahkalroodi , Shihreh Zare Karizi * |
| Islamic Azad University_Varamin-Pishva Branch |
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Abstract: (2610 Views) |
| DNA extraction is a routine step in many biological studies and variety of methods have been established to isolate DNA molecules from biological materials. most methods share three aims: 1- the comprehensive lysis of cells and extraction of intracellular nucleic acids into aqueous solution, 2- the removal of non-nucleic acid organic and inorganic molecules from resultant aqueous extracts and 3- the minimization of nucleic acid losses throughout this purification process. Rainbow trout fish, including salmonid species have been domesticated and today the most important cold-water fish species in the country. To accomplish this study 4 Extraction 1. Extraction Kit Company Gena All 2- Trizol Reagent 3- traditional method of heating and Proteinase K 4- phenol chloroform were studied and It was found that the The best extraction method in terms of quality and the quantity of DNA extracted by 1 percent agarose gel and Nanodrop and Also in terms of the effectiveness of PCR for DNA extraction kit method is the enterprise Gene All Country South Korea. |
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| Keywords: Golden rainbow trout farmed, DNA extraction, Company extraction kit Gene All, PCR. |
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Full-Text [PDF 671 kb]
(1685 Downloads)
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Type of Study: Research |
Subject:
Special
Received: 2018/07/15 | Accepted: 2018/07/15 | Published: 2018/07/15
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