This study was carried out with the aim of measuring steroid hormones levels residue including 17β- estradiol (E₂), 17α- hydroxy progesterone (17α - OHP), testosterone (T) and gonadotropine hormones (GTH I and GTH II) in farmed Persian (Acipenser persicus) and Siberian (Acipenser baerii) sturgeon in International Sturgeon Research Institute (Areo). 10 gram of eggs weighed and centrifuged at 3000 rpm per 10 minutes after homogenizing for extract. Results showed that maximum level of testosterone was 13.2 ± 0.97 ng/ml in Acipenser persicus and showed significant difference compared to Acipenser baerii. Maximum level of progesterone and E₂ were 2.9 ± 0.1 and 1.4 ± 0.6 ng/ml in Acipenser persicus and showed significant difference compared to Acipenser baerii (P<0.05). Based on results, sex steroid hormone residue observed in sturgeon eggs so that steroid hormones levels residue was more than gonadotropin I and II level and was different depended on hormone type and species.