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Effect of Caspian Sea Water Soluble–Fraction of Crude oil on liver antioxidant Enzymes activity as biomarker of oil pollution in Persian sturgeon (Acipenser persicus) juveniles
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Shayan Ghobadi *   , Elahe Khodabakhsh |
| Islamic Azad University-Babol Branch |
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Abstract: (2225 Views) |
| This study evaluate the effects of water soluble fraction of Caspian Sea crude oil on Super oxide dismutase and Catalase enzymes concentration in juvenile Persian sturgeon (Acipenser persicus) average weighting 3.14±0.18 g. Firstly, the LC50 96h value of water soluble fraction of crude oil was calculated (31.94 mg/L) and then the control group and 3 treatments were choosed in lower concentrations (0.25LC50, 0.50LC50 , 0.75LC50) than the LC50 96h. Each experimental group had 3 replicates. Experiments period was 96h and in each 24h the liver were sampled and enzymes concentrations were calculated. During the experimental period physical and chemical factors of water were measured. As the result, the Super oxide dismutase enzyme concentration in third day showed significant difference between all treatments with control (p<0.05). Even if the 0.75LC50 treatment showed significant increase in enzyme concentration (p<0.05). On the other hand the concentration of Catalase enzyme in different treatments had not different signification durin first and second days of experiment (p>0.05), and only the 0.75LC50 treatment showed significant increase in enzyme concentration in third day (p<0.05). In forth day only the 0.75LC50 and the 0.50LC50 treatments showed significant differences with other treatmens (p<0.05). The results showed that the Super oxide dismutase and Catalase enzymes are acceptable biomarkers for oil pollution in Persian sturgeon juveniles, even if because of the delay in increasion of Catalase, the liver Super oxide dismutase is the better biomarker for this pollution in this fish. |
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| Keywords: Persian Sturgeon (Acipenser persicus), Crude oil, Catalase, Super oxide dismutase, Biomarker. |
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Full-Text [PDF 482 kb]
(1167 Downloads)
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Type of Study: Research |
Subject:
Special
Received: 2015/12/4 | Accepted: 2016/06/13 | Published: 2018/09/16
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