This study was done to investigate some hematological and immunological effects of Rhabdovirus carpio, the causative agent of Spring viremia of carp, in Caspian kutum(Rutilus frisii kutum). The challenge study was designed in 3 treatments of control, intra peritoneal injection and bath treatments in triplicates and was monitored for four weeks. For this purpose, 135 pieces of Caspian kutum fry have been challenge to SVC virus. Hematological parameters including total leukocytes (WBC) and erythrocytes counts (RBC), hemoglobin (Hb) content, hematocrits (HCT), leukocytes differential count and blood indices such as MCV, MCH and MCHC were examined. Also immune parameters including cortisol, lysozyme, complement and immunoglobulin was measured. The results of this study showed that the first clinical signs and mortality occurred in 13th and 16th days after challenge in intra peritoneal injection and bath treatments, respectively. Hematological parameters in the bath and intra peritoneal injection treatments compared with the control group significantly increased. The bath treatment showed the highest incresement of hemoglobin, hematocrit, mean cellular hemoglobin concentration (MCH) and mean corpuscular volume (MCV) except the mean cell hemoglobin concentration (MCHC) compared with the intra peritoneal injection treatment (P<005). leukocytes differential count, including percentage of neutrophils, monocytes and eosinophils increased in the bath and intra peritoneal injection treatments than the control group. But the bath treatment showed the highest rate of increasement of neutrophils, monocytes and eosinophils compared with the intra peritoneal injection in (P<005). Percentage of lymphocytes in the bath and intra peritoneal injection treatments was lower than the control. The lowest rate was observed in the bath treatment compared to intra peritoneal injection. The immune parameters included Cortisol, lysozyme, Immunoglobulins and Complement system showed significant increase in bath and intra peritoneal injection treatments than control group. The highest increase of immune parameters was observed in intra peritoneal injection treatment than bath treatment except the complement system (P<0.05). Regarding to results it could be concluded SVC virus able to make changes hematological and immunological parameters Followed by Clinical symptoms and mortality in Caspian kutum fry.