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Population genetic structure of Silurus glanis in Anzali lagoon and Aras lake using microsatellite markers
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Azar Amiri *   , ْShahram Behmanesh2 , Fereidon Chakmehdouz Ghasemi2 , Saber Zahri3 |
Department of Biology, Faculty of Fisheries Sciences, Ahvaz branch, Islamic Azad University, Ahvaz, Iran, Azad Islamic University , chakmehdouz13@yahoo.com
2- Inland Waters Aquaculture Research Center
3- Azad Islamic University |
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Abstract: (3199 Views) |
In this article the population genetic structure of the European catfish (Silurus glanis) in the Anzali lagoon and Aras Lake was investigated based on the characterization of microsatellite markers. For this purpose, sixty fin clip samples of this species from the two regions were collected and for genetic analysis 8 microsatellite loci were used that 2 sets of them produced monomorphic and 6 polymorphic bands. Number of alleles, observed and expected heterozygosity values for each population, population genetic differentiation based on AMOVA test (Fst), genetic distance and Hardy-Weinberg equilibrium test (HWE) between samples of two areas were determined using Biocapt and GenAlex software packages. Totally 88 alleles were observed. The mean number of alleles in samples of Anzali lagoon and Aras lake were 9.3 and 5.3 respectively. There were significant differences based on average number of alleles per locus and heterozygosities between two populations (P< 0.01). The value of Fst between populations was high (0.165) and genetic distance was 0.38 and both of computed parameters were significant (P<0.01). Excess or lacks of heterozygosity was seen but most of used microsatellite loci in two areas were at Hardy-Weinberg equilibrium. The result of this study showed the two population are genetically differentiated, so fisheries management for reproduction and breeding of these populations to export and exchange access is recommended.
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| Keywords: Silurus glanis, Anzali lagoon, Aras lake, Microsatellite DNA markers |
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Full-Text [PDF 605 kb]
(740 Downloads)
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Type of Study: Research |
Subject:
Special
Received: 2015/06/14 | Accepted: 2015/11/20 | Published: 2018/05/25
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