1 2322-3545 معاونت پژوهش و فناوری دانشگاه آزاد اسلامی واحد لاهیجان 534 Special Effect of Nitrogen deficiency and light intensity on the fatty acid composition of Chaetoceros calcitrans akbarnejad mahsa b rajabi islami houman c javaheri baboli mehran d shamsaie mehrgan mehdi e filizadeh yousef f b Department of Fisheries, Science and Research Branch, Islamic Azad University, Tehran, Iran. c Department of Fisheries, Science and Research Branch, Islamic Azad University, Tehran, Iran. d Department of Fisheries, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran. e Department of Fisheries, Science and Research Branch, Islamic Azad University, Tehran, Iran. f Department of Agriculture, Agronomy and Plant Breeding,ShahidChamranUniversity, Ahvaz, Iran. 1 1 2020 13 4 1 12 17 01 2018 11 05 2018 Marine diatoms are important sources of fat and fatty acids that are considered for aquaculture and alternative energy sources, and under varying environmental conditions in algae. In order to influence the nitrogen deficiency and light intensity of the medium culture on the C.calcitrans fatty acid composition, algae was exposed to 4 nitrogen treatments (25,50,75 and 100% nitrogen) and light intensity (80, 160, 240 µmol m-2 s-1) in 1 liter Erlenmeyer was cultured. Sampling to determine fatty acid composition was done by harvesting in stationary phase and by Gas-chromatography. The results showed that total saturated fatty acids, C14:0  (Myristic acid), C16:0 (Palmitic acid), and mono unsaturated fatty acids, C16: 1n-7(Palmitoleic acid), C16: 1n-9(Hexadecenoic acid) and C18: 1n-9 (Oleic acid) were decreased by enhancing media nitrogen concentration (P≤0.05). Poly unsaturated fatty acids and also EPA and DHA fatty acids by enhancing nitrogen concentration were increased. Saturated fatty acids, C16:0 (Palmitic acid), C18:0 (Stearic acid), mono unsaturated fatty acids and C16: 1n-7 increased significantly with light intensity enhancement (P≤0.05). Poly unsaturated fatty acids did not show significant difference with light intensity enhancement. Also, the amount of DHA fatty acids did not show significant changes with light intensity, but the EPA increased significantly in the treatment of (240 µmol m-2 s-1) compared to 2 showed another light intensity (80 and 160 m-2 s-1). The overall results showed that unsaturated fatty acids increased with increasing nitrogen concentration in the medium culture, but did not increase fatty acids with increasing light intensity. Conversely, with increasing nitrogen concentration, saturated fatty acids decreased and with increasing light intensity saturated fatty acids increased.
603 Special Effect of methanol extract of gokshura (Tribulus terrestris( on liver and digestive enzymes and blood biochemical parameters in grey mullet, Mugil cephalus Linnaeus 1758 akbary paria g daliran simin h g Chabahar Maritime University h Chabahar Maritime University 1 1 2020 13 4 13 25 27 12 2018 08 04 2019 This experiment was conducted to evaluate the effect of methanol extract of gokshura (Tribulus terrestris( (MET) on liver and digestive enzymes and blood biochemical parameters in grey mullet, Mugil cephalus for 60 days. The experiment was conducted in a completely randomized design with 240 grey mullet (with average weight of 8.42±0.43g  and average length of 6.45± 0.40 cm) in 4 treatments and 3 replicates (n=10 in each replicate) and included:  treatment 1 (control), treatment 2 (0.5g MET/kg diet), treatment 3 (1g MET/kg diet) and treatment 4 (1.5g MET/kg diet). At the end of experiment, the lowest cholestrol and triglyceride and and highest albumin of blood serum were observed in treatment 3 and 4 (P< 0.05). The lowest glucose androtein and glubolin  were observed in treatment 4 and there is a significant difference between all of treatments (P< 0.05). The highest the activity of amylase  enzyme and the lowest the activity of asoartat aminotransferase and alanin aminotransferase enzymes were observed in treatment 3 and 4 but there was no significant difference among them (P>0.05). So, finaly the present results showed that use of the diet containing 1.5 g g  the methanol extract o of gokshura /kg had positive effects on the activity of liver and digestive enzymes and blood biochemical parameters of grey mullet.   442 Special Effect of herbal supplement of Sangrovit on growth performance, blood biochemical parameters, survival and stress resistance in Oncorhynchus mykiss fry Imanpoor1 Mohammad Reza i Zahra Roohi Zahra j Imanpoor Sara k i Fisheries Sciences, Department of Fisheries and Environment, Gorgan University of Agricultural Sciences and Natural Resources, Iran. j Fisheries Sciences, Department of Fisheries and Environment, Gorgan University of Agricultural Sciences and Natural Resources, Iran. k Fisheries Sciences, Department of Fisheries and Environment, Gorgan University of Agricultural Sciences and Natural Resources, Iran. 1 1 2020 13 4 27 36 19 01 2017 07 05 2017 This study was conducted to evaluate the effect of herbal supplement of Sangrovit on the growth performance, blood biochemical indices, survival and resistance to salinity stress in rainbow trout fry. Fish (1.509±0.011 g) were divided into four groups fed on diets containing Sangrovit in different levels: 0 (control), 0.05, 0.1 and 0.15 % for 45 days. Results showed that specific growth rate and body weight gain were significantly (P<0.05) different among the experimental groups, and highest levels of them were observed in treatment groups with Sangrovit. Feed conversion ratio and total protein decreased significantly in fish fed diets containing Sangrovit. On the other hand, there were no significant differences were observed in glucose and cholesterol levels among the treatments (P>0.05). Blood samples were obtained in four times after stress (first, third, fifth and seventh days) for evaluate hematocrit levels. Salinity significantly affected hematocrit levels (P<0.05). On the first day of after stress, the hematocrit levels significantly were increased in all groups (P<0.05). However, the hematocrit levels were decreased gradually in all groups since third day. Present study suggests that Sangrovit at 0.1% can effectively improve the growth performance and feed utilization in rainbow trout fry. 633 General Effect of different levels of Aloe vera powder on growth performance and hematological indices of goldfish (Carassius auratus) hosennezhad jadidi maryam l khoshkholgh majidreza m alaf noveyrian hamid n l Faculty of Natural Resources, University of Guilan m Faculty of Natural Resources, University of Guilan n Faculty of Natural Resources, University of Guilan 1 1 2020 13 4 37 53 07 05 2019 11 07 2019 The aim of this study was to investigate the addition of different levels of aloe vera powder to diet and its effect on growth performance and blood indexes of goldfish. 120 fish with an average initial weight of    75.7 ± 0.03 g were fed for 56 days with diets containing 0, 5, 10 and 15 g/kg aloe vera powder. The result of growth indices showed that final weight increased in 5 and 10 g/kg treatments and was significant different with 15 g/kg treatment and control group respectively (P<0.05). Weight gain, weight gain percentage, specific growth rate and feed conversion ratio was different in 10 g/kg treatment with other experimental groups (P<0.05). RBC, hemoglobin and hematocrit percent increase in 5 g/kg treatment and were different with 15 g/kg treatment and control group (P<0.05). WBC and neutrophil percentage increased in 5 and 10 g/kg treatment and were different with 15 g/kg treatment and control group (P<0.05).  Also, the highest percentage lymphocytes was observed in 10 g/kg treatment, which was different from 15 g/kg treatment and control group (P<0.05). Accordingly, the addition of aloe vera powder is recommended, especially at a level of 10 g/kg of goldfish diet. 622 Special Feeding Western white shrimp (Penaeus vannamei Boone, 1931) with bioflocs biomass and its effects on water quality and growth performance khanjani mohammad hossein o Sajjadi Mir Masoud p Alizadeh Morteza o university of jiroft p Guilan University 3. Saline Water Aquatic National Research Cen*ter 4. Department of Fisheries, Faculty of Marine Science and Technology, University of Hormozgan, Bandar Abbas, Iran 1 1 2020 13 4 55 67 27 03 2019 11 07 2019 The effect of feeding Western white shrimp Penaeus vannamei Boone, 1931 (in three stage post­larvae, nursery and juvenile) with bioflocs biomass on water quality and growth performance were investigated in the present work. Western white shrimps were fed (with wet biofloc) for three weeks in fiberglass containers with 180 liters volume of water and density of 10 post larvae per liter (post­larvae), 1 individual per liter (nursery) and 1 gr biomass per liter (juvenile) in three biofloc treatments at different feeding levels of 25, 15 and 6% of body weight. The results obtained in this study allowed, Water quality parameters including temperature, salinity, dissolved oxygen and pH, no significant differences were observed among the biofloc treatments (P>0.05). Maximum dissolved oxygen (6.34 ± 0.31) and maximum pH (8.33 ± 0.05 mg/lit) was in biofloc post­larvae treatment. Maximum and minimum level of ammonia was 0.34± 0.21 and 0.1± 0.08 mg/lit in biofloc juvenile and post­larvae treatments, respectively and showed significant difference between treatments (P<0.05). The maximum growth rate (50± 10 mg per day), specific growth rate (10.92± 1.37 %/day) and survival rate (%96.29± 0.76) were observed in biofloc treatments, respectively (juvenile, post­larvae and nursery). Also, the highest feed conversion ratio (4.11±1.08) and the lowest feed efficiency (%24.28±6.38) were obtained in juvenile biofloc treatment. The results showed that the presence of biofloc improved water quality. Western white shrimp grown with feeding of bioflocs biomass but no sufficient food source (only biofloc) for growth and production.   547 Special The effects of singular or combined administration of Pediococcus acidilactici and Raffinos on growth performance and some biological characteristics of eggs and spermatogonal parameters in gold fish (Carassius auratus) sepehfar delara hoseinifar seyed hosein Jafarnodeh ali Department of Fisheries, Faculty of natural resource, Urmia University, Urmia, Iran Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran. Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran 1 1 2020 13 4 69 79 15 03 2018 28 05 2018 Summary The present study investigated the effect of singular or combined administration of Pediococcus acidilactici and Raffinos on growth performance and some biological characteristics of eggs and spermatogonal parameters of gold fish (Carassius auratus). 180 gold fish, with average weight of 26.3±0.18 after two weeks of adaptation to laboratory condition In 4 treatments and each treatment with 3 replicates including: commercial diet (control group), commercial diet supplemented with 0/9×107 CFU/g P. acidilactici, 10 g kg-1 Raffinos or simultaneously supplemented with 0/9×107 CFU/g  P. acidilactici and 10 g kg-1 Raffinos accidentally divided. The trial lasted 60 days, and fish sampling was performed to evaluate the growth indices and quality indices of egg and sperm at the end of the experiment. The results of analysis of growth indices showed that the rate of body weight gain, weight gain and specific growth rate in synbiotic treatment had a significant difference with the control group (P<0.05). However, there was no significant difference in the level of feed conversion ratio, condition factor and survival index in different treatments (P> 0.05) Also, the analysis of oocyte and sperm quality indices showed that there was no significant difference in the level of sperm density, spermatocrit and pH of seminal fluid in different treatments (P< 0.05) However, sperm motility duration, oocyte diameter and yolk space were significantly different in the control group (P <0.05). 591 Special Frequency of zooplanktons in complex ponds of reproduction and reconstruction of Shahid Beheshti Aquatic Resources in Rasht and their relationship with physical and chemical factors of water Ebadi Abkenar Zahra Bahri Amirhushang Ramezanpur Zohreh Mohammadizadeh Flora Shenavar Masole2 Ali Reza Islamic Azad University,Bandar Abbas Branch Islamic Azad University,Bandar Abbas Branch International Sturgeon Research Institute Islamic Azad University,Bandar Abbas Branch International Sturgeon Research Institut 1 1 2020 13 4 81 91 09 11 2018 08 11 2019 Abstract. Zooplanktons are important elements of an aquatic ecosystem such as ponds. They are involved in rotation of materials, control of algae communities and microbial production. The aim of this study was to investigate the frequency and identification of zooplankton in sturgeon fish ponds of Shahid Beheshti aquatic reproduction and reconstruction complex and their relation with physical and chemical factors of water. Five ponds were studied. Physical factors such as dissolved oxygen, temperature, pH and saturation, and chemical factors such as nitrite, nitrate, ammonia and orthophosphate were measured twice a week with 3 replicates. A total of 22 genera were identified from 4 branches of Cladocera (5 genera), Copepoda (3 genera), Protozoa (2 genera) and Rotifera (12 genera) in the studied ponds. The highest variety and abundance were found in Rotifera and Copepoda respectively. Nauplius, Daphnia and Cyclops had the highest percentage of zooplankton with 46%, 25% and 15% respectively. The results of measurement of physical and chemical factors of water indicated optimal water conditions of the ponds and only the orthophosphate factor was higher than the limit. There was a significant negative correlation between pH and orthophosphate factors (P> 0.05). There was a significant positive correlation between cladocera and orthophosphate factor as well as zooplankton and nitrate factor in all ponds (P<0.01). Based on the results, the ponds were in optimum condition in terms of physical and chemical factors and zooplankton population and only the orthophosphate factor was above the permissible limit which could be controlled by proper management. 628 Special Influence of light on development of digestive tract process and some indices of growth in Persian sturgeon (Acipenser persicus) larvae Kazemi Rezvanollah Firoozbakhsh Farid Hallajian Ali Gholami Sara International Sturgeon Research Institute, Agricultural Research Education and Extension Organization (AREEO), Rasht, Iran Animal Science and Fisheries Faculty of Agricultural and Natural Science University of Sari International Sturgeon Research Institute, Agricultural Research Education and Extension Organization (AREEO), Rasht, Iran Animal Science and Fisheries Faculty of Agricultural and Natural Science University of Sari 1 1 2020 13 4 93 106 15 04 2019 13 09 2019 The aim of this study was to access the basic information of digestive tract and growth indices of Persian sturgeon (Acipenser persicus) larvae at endo feeding stage (8 days) under optical interaction between photoperiod and light intensity. This research was carried out with 7 treatments including 4 light intensities (0, 50, 150 and 300 lux) and three photoperiods of 24 hours lighting and without darkness (24L:00D), 12 hours lighting and 12 hours darkness (12L:12D), 24 hours Dark and non-lighting (00L:24D) and control group which each treatment having three replications. The larval digestive tract was sampled on a daily basis. Histological specimens of the larval digestive tract were prepared with 7μm slides after fixed in Buen's solution and tissue processing based on standard histological methods. The slides were stained with Hematoxylin and Eosin (H & E), and they were studied with a light microscope. Also, growth indices including final weight, specific growth factor and larvae weight gain were calculated at the end of the experiment. The results of this study showed that the process of development the digestive tract of larvae, which were affected the higher light intensity (150-300 lux) and longer photoperiod (12-24 hours), were faster and more balanced than other treatments. Growth indices were also significantly better in the same treatments. So, Persian sturgeon larvae require proper and simultaneous management of photoperiod and light intensity for optimal growth in the endo nutrition stage.   629 Special Prioritization of Allium sativum, Mentha piperita and Oregano vulgare extracts on improving growth and immune indices of reared giant sturgeon (Huso huso) Nikbakhsh Bidarouni Abbas Vahabzadeh Roudsari Habib Islamic Azad University Islamic Azad University 1 1 2020 13 4 107 121 19 04 2019 17 09 2019 Applying herbal food attractant, in addition, to encourage fishes to food intake tends to promote growth indices and also stimuli the immune system and finally decreases antibiotic consumption and environmental tension. In this survey, the effects of Allium sativum, Oregano vulgare and Mentha piperita extracts on growth indices, survival rate and health indicators of reared giant sturgeon (Huso huso) juvenile were conducted. Sixty specimens of Huso huso with an average weight of 360 ± 2.25gr. Were supplied from Research Centre of Dr. Keyvan, stocked in 12 tanks with 350 liters water volume, four treatments with triplicates (5 specimens in each replicate) designed for this experiment. Fishes fed by 1.2% of biomass 4 times daily by extruded food supplement including 0.1 gr/kg of Mentha piperita and 0.1 gr/kg Mentha pulegium and 0.5 gr/k Allium sativum extracts during a 60 days experiment. Biometric measurements were done every 15 days, the mean value of GR, SGR, FCR, K factor measured based on standard methods. Immune parameters evaluated by blood samples. FCR of Mentha pulegium and Mentha piperita contained diets were significantly lower than Allium sativum treatment (P<0.05). The mean value of GR, SGR and K factor in Allium sativum treatment were significantly low (P<0.05) while these factors in Mentha piperita and Mentha pulegium treatments were significantly high (P<0.05). Results showed that the number of white blood cells, red blood cells, hemoglobin, hematocrit, mean corpuscular volume (MCV), mean corpuscular concentration (MCH), the average MCHC, neutrophils, lymphocytes, eosinophils, lysosomes, IgM and ALP in Huso huso blood had no significant difference in all treatments (P>0.05). Levels of ALT and AST in blood serum of fishes fed diet contained Allium sativum were significantly higher than other treatments (p<0.05). Overall, the use of 0.1 gr/k of the extracts of Mentha piperita and Mentha pulegium in the diet improved growth indicators, while no growth indices improvement observed by using 0.5 gr/k of Allium sativum extract. None of the treatments had effects on the non-specific immune (cellular or humoral immunity) abilities. Although no statistical difference observed in the survival rate of treatments. According to the results, priority use of herbal additives for juvenile Huso huso is Mentha pulegium, Mentha piperita and Allium sativum.   581 Special Evaluation of Total Factor Productivity and Efficiency of Rainbow Trout (Oncorhynchus mykiss) Marine Cage Farms in the Mazandaran Province Yazdani Saeed Rafiee Hamed Ramezani Mohammadreza University of Tehran University of Tehran University of Tehran 1 1 2020 13 4 123 134 30 09 2018 02 03 2019 Fish cage culture is one of the systems of fish farming which has been considered in many countries including Iran, due to its high advantages. Purpose of any economic activities is to maximize profits. Considering the facilities and constraints in the agricultural sector of Iran's economy, the most appropriate way to increase the production and income of farmers is to use of optimal and proper level of production factors. The aim of this study is to evaluate the total factor productivity and efficiency of fish cage farms in Mazandaran province. Required data were collected through interviews and a detailed questionnaire. Based on the results, the total factor productivity index of 4 out of 9 farms was less than 1 and the average of this index was 0.998. On the other hand, the averages of technical, allocative and economic efficiency were 0.884, 0.725 and 0.645, respectively. The results showed that technical efficiencies of three farms were equal to one, and the allocative and economic efficiency of just 1 out of 9 farms was equal to one. Inefficiencies of farms in use of inputs were too drastic that if the cost minimizing input quantities had been used and the proper management had been applied, they would have been able to achieve the current level of their productions at 40% less cost. Given the emergence of fish cage culture in Iran, it is suggested that successful farms be encouraged by incentive policies to transfer their experiences to other farms in order to improve productivity and efficiency. This can be done through training courses using well trained instructors and successful farmers.