معاونت پژوهش و فناوری دانشگاه آزاد اسلامی واحد لاهیجان
Journal of Aquaculture Development
2322-3545
10
4
2017
1
1
Subacute Effects of copper oxide nanoparticles on some hematological indices and gill tissue of the juvenile carp(Cyprinus carpio)
1
14
FA
H.
Ahmadi
Department of Biology, Faculty of Science Faculty of Science, Guilan University
A.S
Naeemi
Department of Biology, Faculty of Science Faculty of Science, Guilan University
F.
Nazarhaghighi
Young Researchers and Elite Club, Rasht Branch, Islamic Azad University, Rasht
H.
Ghafuri
Department of Biology, Faculty of Science Faculty of Science, Guilan University
Technological and industrial development in recent decades led to large inputs of pollutants to the environment. Due to the harmful effect of pollutants on the environment and living organisms, investigate and prove the damage caused by them is necessary. The aim of this research was to study the pobable toxicity of Cuo nanoparticles (NPs) in the juvenile Cyprinus carpio by evaluating of hematological parameters and histopathological effects. Juvenile carps were treated in 12 aquariums containing the sublethal concentrations of 50, 100 and 200 mg.L-1 of CuO NPs and a control group (without CuO NPs) in three replicates. Fish were exposed to CuO NPs for 14 days. Blood samples were collected after 7 and 14 days and hemoglobin (Hb), hematocrit (Hct), red blood cells (RBC), white blood cells (WBC), lymphocytes, monocytes, eosinophil and neutrophils were measured. Also, MCV, MCH and MCHC were calculated. Tissue sections were prepared from fish gills. Tissue damage was evaluated. The analysis of hematological indices showed that CuO NPs significantly reduced the RBC, Hb, Hct (in 100 and 200 ppm), MCH (in 100 ppm) and MCV (in 50 and 200 ppm) after 14 days (P< 0.05). CuO NPs significantly affected the counts of lymphocytes, monocytes and neutrophils (P< 0.05). The results of gill histopathology showed alterations such as hyperemia, hemorrhage, hyperplasia and adhesion with high intensity and destroyed primary filaments, cell necrosis and telangiectasia with less intensy. Thus it can be concluded that copper oxide nanoparticles at concentrations examined in this study had negative effects on Juvenile carp.
معاونت پژوهش و فناوری دانشگاه آزاد اسلامی واحد لاهیجان
Journal of Aquaculture Development
2322-3545
10
4
2017
1
1
The effects of salinity on some of plasma biochemical factors and intestinal tissue of Asian sea bass (Lates calcarifer)
15
26
FA
s.
Aashraf
Department of Aquaculture, Bushehr Branch, Islamic Azad University, Bushehr
A.
Pazira
Department of Aquaculture, Bushehr Branch, Islamic Azad University, Bushehr
M.
Nafisi Bahabadi
Department of Fisheries, Persian Gulf University. Persian Gulf Research Center, Bushehr
The effect of different salinity levels (15, 35, 50 ppt) and Freshwater on biochemical parameters and intestinal tissue of Lates calcarifer was surveyed for 30 days. This experiment in a completely randomized design with 4 different treatments Freshwater and salinity levels (15, 35, 50 ppt) and treated with 3 replicates and 15 fishes (initial weight 32gr) were used for each repetition. In this study some blood biochemical parameters (Sodium, Potassium, Chloride, Osmolality) was measured at the end of the experiment. The analysis results of blood biochemical parameters showed no significant differences between the treatments (P>0.05). This study also covers intestine histology of Lates calcarifer in 4 treatments was investigated and the results showed that the digestive system has normal villi and enterocyte and there was no significant difference in the location of the cell nucleus in the various histological slides. The images from different treatments were observed no histological lesions. The structure of mucous layers, muscular, serous and intestinal mucosa cells was normal and did not show significant differences. No significant difference in the results show that this fish is a species resistant to salinity environments hyperhaline tolerated.
معاونت پژوهش و فناوری دانشگاه آزاد اسلامی واحد لاهیجان
Journal of Aquaculture Development
2322-3545
10
4
2017
1
1
The effect of Rhabidovirus carpio on hematological and immune parameters of Caspian Kutum (Rutilus frisii kutum)
27
38
FA
S.
Omidvar
Inland Waters Aquaculture Research Center, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Bandar Anzali
Mohades
Ghasemi
Inland Waters Aquaculture Research Center, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Bandar Anzali
Z.
Omidvar
Gonbad Kavous University, Faculty of Agriculture and Natural Resources Golestan
S.
Haghighi Karseydani
Department of Fisheries, Bandar Anzali Branch, Islamic Azad University, Bandar Anzali
K.
Zolfinejad
Inland Waters Aquaculture Research Center, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Bandar Anzali
This study was done to investigate some hematological and immunological effects of Rhabdovirus carpio, the causative agent of Spring viremia of carp, in Caspian kutum(Rutilus frisii kutum). The challenge study was designed in 3 treatments of control, intra peritoneal injection and bath treatments in triplicates and was monitored for four weeks. For this purpose, 135 pieces of Caspian kutum fry have been challenge to SVC virus. Hematological parameters including total leukocytes (WBC) and erythrocytes counts (RBC), hemoglobin (Hb) content, hematocrits (HCT), leukocytes differential count and blood indices such as MCV, MCH and MCHC were examined. Also immune parameters including cortisol, lysozyme, complement and immunoglobulin was measured. The results of this study showed that the first clinical signs and mortality occurred in 13th and 16th days after challenge in intra peritoneal injection and bath treatments, respectively. Hematological parameters in the bath and intra peritoneal injection treatments compared with the control group significantly increased. The bath treatment showed the highest incresement of hemoglobin, hematocrit, mean cellular hemoglobin concentration (MCH) and mean corpuscular volume (MCV) except the mean cell hemoglobin concentration (MCHC) compared with the intra peritoneal injection treatment (P<005). leukocytes differential count, including percentage of neutrophils, monocytes and eosinophils increased in the bath and intra peritoneal injection treatments than the control group. But the bath treatment showed the highest rate of increasement of neutrophils, monocytes and eosinophils compared with the intra peritoneal injection in (P<005). Percentage of lymphocytes in the bath and intra peritoneal injection treatments was lower than the control. The lowest rate was observed in the bath treatment compared to intra peritoneal injection. The immune parameters included Cortisol, lysozyme, Immunoglobulins and Complement system showed significant increase in bath and intra peritoneal injection treatments than control group. The highest increase of immune parameters was observed in intra peritoneal injection treatment than bath treatment except the complement system (P<0.05). Regarding to results it could be concluded SVC virus able to make changes hematological and immunological parameters Followed by Clinical symptoms and mortality in Caspian kutum fry.
معاونت پژوهش و فناوری دانشگاه آزاد اسلامی واحد لاهیجان
Journal of Aquaculture Development
2322-3545
10
4
2017
1
1
Effects of commercial probiotic Lactobacillus on survival and growth parameters and nutritional indexes of common carp (Cyprinus carpio)
39
49
FA
ّF.
Baesi
Department of Fisheries, Faculty of Natural Resources, Behbahan Khtam Alanbia University of Technology, Behbahan
A.
Aberomandu
Department of Fisheries, Faculty of Natural Resources, Behbahan Khtam Alanbia University of Technology, Behbahan
S.
Ziaee nejad
Department of Fisheries, Faculty of Natural Resources, Behbahan Khtam Alanbia University of Technology, Behbahan
Mehran
Javaheri Baboli
Department of Fisheries Science, Ahvaz Branch, Islamic Azad University, Ahvaz
The purpose of this study was to evaluate the effect of probiotic Lactobacillus trade on growth parameters, survival and nutrition indices of common carp (Cyprinus carpio). The 243 fish pieces were put with weight average of 53.39±5.78 in 9 tanks of 300 liter fiberglass with a volume of 250 liters of water for 8 weeks using three treatments contain zero (control), 103 CFUg-1 and 106 CFUg-1 lactobacillus probiotic were grown commercially. The project was carried out in 3 treatments with 3 replications. Fish feed were done with two times in day and 2% body weight The after finish the experiment, fishes growth parameters, survival and nutrition indicators were examined. The obtained results showed that the specific growth rate, final weight, feed efficiency, protein and fat-performance ratio in the tested group of treatment 106 CFUg-1 probiotics was higher than other. CFUg-1 103 probiotic treatment had the highest survival rate but feed conversion efficiency and feed conversion ratio was the lowest. The condition factor and liver and visceral indexes of the treatments showed no significant difference with control group.
معاونت پژوهش و فناوری دانشگاه آزاد اسلامی واحد لاهیجان
Journal of Aquaculture Development
2322-3545
10
4
2017
1
1
Effect of Enriching Artemia franciscana with Unsaturated Fatty Acid and Vitamin E on Growth, Survival and Resistance to Temperature and Salinity Stress of Sepia pharaonis Larvae
51
61
FA
M.R
Zahedi
Department of Fishery, Bandar Abbas Branch, Islamic Azad University, Bandar AbbasوPersia Gulf And Oman Sea Ecological Research Institute, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Bandar Abbas
A.H.
Bahri
Department of Fishery, Bandar Abbas Branch, Islamic Azad University, Bandar Abbas
M.
Yahyavi
Department of Fishery, Bandar Abbas Branch, Islamic Azad University, Bandar Abbas
F.
Mohamadi zadeh
Yung Researches and Elite Club, , Bandar Abbas Branch, Islamic Azad University, Bandar Abbas
M.
Yasemi
Associate of Applied Sciences Institute of Agricultural Jihad Organization of research, education and extension (AREEO), Tehran
Abstract: To evaluate the effects of using Artemia fransiscana enriched with cod liver oil and vitamin E on growth, survival and salinity and thermal stress resistance of Sepia pharaonis larvae this study was conducted. 25 days after hatching the larvae of Sepia pharaonis larvae with average length of 15.97±0.15 mm, 3.53±0.03 gr body weight transferred into culture tanks in five treatments (each with 3 replicates). Experimental treatments include: Artemia fransiscana enriched with cod liver oil at different levels of E1= 5%, E2= 10% and E3=15 % vitamin E , non-vitamin cod liver oil, and control (non-enriched artemia) group. . In each replicate, the 20 larvae were introduced into tanks of 20 liters and 10 artemia /liter/ day to feed larvae were used. At the end of experiment, To investigate stress resistance, at the end of experiment three groups of larvae were encountered at one hour stress with 5, 15, 25 ppt salinity , and 10, 25 ,32 Ċ temperature levels. The results showed that feeding with vitamin E elevate survival and growth factors of Sepia pharaonis larva, But did not show a significant difference with control (P > 0.05), In the event that HUFA treatment showed a significant difference with other groups Statistically(P < 0.05). The results of this research showed that unsaturated fatty acids can improve growth and survival in cuttlefish larvae, but vitamin E did not exhibit any effect on survival rate, growth and stress resistance (P >0.05).
معاونت پژوهش و فناوری دانشگاه آزاد اسلامی واحد لاهیجان
Journal of Aquaculture Development
2322-3545
10
4
2017
1
1
Population genetic structure of Caspian roach (Rutilus rutilus caspicus) using DNA sequencing in South Caspian Sea and Aras Lake
63
73
FA
M.
Sharifi
Department of Marine Biology, North Tehran Branch, Islamic Azad University, Tehran
P
Negatkhah Manavi
Department of Marine Biology, North Tehran Branch, Islamic Azad University, Tehran
F.
Chakmehdouz Ghasemi
Iranian Fisheries Science Research Institute, Inland Water Aquaculture Research Center, Agricultural Research Education and Extension Organization (AREEO), Bandae Anzali
Sh.
Behmanesh
Iranian Fisheries Science Research Institute, Inland Water Aquaculture Research Center, Agricultural Research Education and Extension Organization (AREEO), Bandae Anzali
معاونت پژوهش و فناوری دانشگاه آزاد اسلامی واحد لاهیجان
Journal of Aquaculture Development
2322-3545
10
4
2017
1
1
Eeffec of C/N different ratios on water quality parameters and growth performance of common carp (Cyprinus carpio) fingerlings in biofloc system
75
89
FA
A.
Azimi
Department of Fisheries, Faculty of Agriculture and Natural Resources, Gonbad Kavous University, Gonbad Kavous
H.
Jafaryan
Department of Fisheries, Faculty of Agriculture and Natural Resources, Gonbad Kavous University, Gonbad Kavous
M.
Harsij
Department of Fisheries, Faculty of Agriculture and Natural Resources, Gonbad Kavous University, Gonbad Kavous
H.
Gholipour
Department of Fisheries, Faculty of Agriculture and Natural Resources, Gonbad Kavous University, Gonbad Kavous
R.
Patimar
Department of Fisheries, Faculty of Agriculture and Natural Resources, Gonbad Kavous University, Gonbad Kavous
A study in order to evulated to do the effects of different levels of organic carbon supplement on biofloc formation, inorganic nitrogen and phosphat situation, feed efficiency and growth performance of common carp (Cyprinus carpio) in biofloc system for 42 days. After of acclimatization period, 180 common carp fingerlings with (weight average ± SD, 5/78 ± 0/10 g) were stocked in roundel tank with water capacity of 40 l. Determain of C/N different ratios 15:1 (CN-15) and 20:1 (CN-20) were determined by the content of C/N in commercial feed and the amount of carbon in wheat meal that it was daily used to experimental treatments. Wheat meal don’t added to control treatment (≈ C/N ratio, 10:1). Increasing of carbon to nitrogen ratio was increased water quality parameters such as turbidity, total suspended solid (TSS), total dissolve solid (TDS), Biochemical oxygen demand (BOD), alkalinity and nitrat concentration, but it was decreased total ammonia nitrogen (TAN) and phosphat during the experimental period (p<0/05). Water exchange was reduced 50 % in biofloc treatments than control. Growth performance of common carp fingerlings in different treatments indicated that the fingerlings of CN-15 treatment had the highest of body wieght, specific growth rate, protein efficiency rate (PER) and the lowest of feed conversion rate then both of CN-20 and control treatments. The results of this study suggest that carbon to nitrogen ratio 1:15 a positive impact on water quality parameters, improve food consumption and growth performance of common carp in biofloc system and reduces the amount of water Consumption in the system.
معاونت پژوهش و فناوری دانشگاه آزاد اسلامی واحد لاهیجان
Journal of Aquaculture Development
2322-3545
10
4
2017
1
1
Planktonic flora and the role of its on yield of silver carp (Hypophthalmichthys molitrix) in warm water fish pond
91
102
FA
A.
Ghelichi
Department of Fisheries, Azadshahr Branch, Islamic Azad University, Azadshahr
M.
Jafari
Department of Fisheries, Azadshahr Branch, Islamic Azad University, Azadshahr
M.
Kamali Sanzighi
Yung Researches and Elite Club, , Azadshahr Branch, Islamic Azad University, Azadshahr
معاونت پژوهش و فناوری دانشگاه آزاد اسلامی واحد لاهیجان
Journal of Aquaculture Development
2322-3545
10
4
2017
1
1
Effect of dietary β-carotene supplementation on some oxidative status biomarkers in common carp (Cyprinus Carpio) tissues
103
111
FA
M.
Mohebbi Moghaddam
Department of food Hygiene and Aquatic Animal Health, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad
H.
Baghshani
Department of Basic Sciences, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad
D.
Shahsavani
Department of food Hygiene and Aquatic Animal Health, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad
The aim of this study was to investigate the possible protective effect of β-carotene on lipid and protein oxidation in carp meat during different storage times. 75 fish (approximately 60g) were randomly divided into three groups of 25 each. Group 1 as control group was fed with basic diet. Group 2 and 3 received 50 and 100 mgkg-1 diet β-carotene, respectively. After the end of study (6 weeks), 10 fish from each group were randomly caught and after removing the viscera and washing were stored at 4°C for 4 days and were sampled at different times (0, 24, 48, 72 and 96 hours) after the harvest. Based on the present study results, muscle malondialdehyde concentrations were significantly lower following β-carotene consumption at 0,24,72 and 96 hours after harvest in the group 3 compared to the control group (p<0/05). Muscle protein carbonyl contents showed a significant decrease following β-carotene consumption in the samples at 0, 48 and 96 hour after harvest in the group 3 compared to the control group. FRAP values were significantly increased following β-carotene consumption at 72 and 96 hours after harvest in the group 3 compared to the control group. The results of the present study indicated that β-carotene as a natural antioxidant is largely effective to improve the oxidative status of carp meat by reducing lipid and protein oxidation.
معاونت پژوهش و فناوری دانشگاه آزاد اسلامی واحد لاهیجان
Journal of Aquaculture Development
2322-3545
10
4
2017
1
1
Effects of fasting on body electrolytes in kuttum fish (Rutilus frisii kuttum) during sea water adaptation
111
124
FA
M.
Mohiseni
Department of fisheries, Faculty of Natural Resources, Behbahan Khatam Alanbia University of Technology
S.M.V
Farabi
Iranian Fisheries Research Organization, Ecology Faculty of the Caspian Sea. Sari
M.
Banaee
Department of fisheries, Faculty of Natural Resources, Behbahan Khatam Alanbia University of Technology
B.
Nematdust Haghi
Department of fisheries, Faculty of Natural Resources, Behbahan Khatam Alanbia University of Technology
Salinity adaptation and sea water tolerance are vital and energy demanding process in the life history of anadromous species such as kuttum fish. As a results of this phenomenon the blood electrolytes fluctuation would be prevented. Evaluation on feed deprivation in ion changes of kuttum fish is the aim of this study. Kuttum juveniles with average weight of 0.5 gram were divided into the couple groups. One group considered as a control and were fed ad libitum during over the experiment and fishes from another group were left fasted. Both groups were exposed to the salinity challenge for 7 days and sampling was done in second, third, fourth and seventh days after challenge initiation. Consequently Na+K+ATPase activity, monovalent (sodium, potassium and chloride) and divalent (calcium) ions were evaluated. Results showed significant decrease in NaKATPase activity as a result of fasting. Some ions (Ca and K) were also decreased significantly. On the other hand, Sodium and chloride were increased in the feed deprived kuttum fish. Based on obtained results we can conclude that feed deprivation may have a disruptive effect on success in seawater adaptation. Therefore more attention should be considered about feeding as an important factor in kuttum recruitment policy.
معاونت پژوهش و فناوری دانشگاه آزاد اسلامی واحد لاهیجان
Journal of Aquaculture Development
2322-3545
10
4
2017
1
1
Study of antibacterial effects of Garlic (Allium sativum) and Zataria multiflora extracts on Aeromonas hydrophila isolated of fish sturgeon
125
132
FA
M.
Masoumzadeh
International Sturgeon Research Institute, Agricultural Research Education and Extension Organization (AREEO), Rasht
M.
Sharif Rohani
Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Tehran
A.
ShenavarMasouleh
International Sturgeon Research Institute, Agricultural Research Education and Extension Organization (AREEO), Rasht
M.
Alizadeh
International Sturgeon Research Institute, Agricultural Research Education and Extension Organization (AREEO), Rasht
J.
Jalilpoor
International Sturgeon Research Institute, Agricultural Research Education and Extension Organization (AREEO), Rasht
S.
Bazari Moghaddam
International Sturgeon Research Institute, Agricultural Research Education and Extension Organization (AREEO), Rasht
Considering bad effects of applying antibiotics and disinfectants which leads to drug resistances in body of aquatic organisms, it is necessary to be replaced with herbal medicine plants that have natural origin.The study aims to examine the effect of the hydro alcoholic extracts Zataria multiflora and garlic (Allium sativum) on Aeromonas hydrophila. In this study after extraction of Allium sativum and Zataria multiflora extracts by percolation method the antimicrobial effects of this extracts were studied on Aeromonas hydrophila by in vitro method . In this study, the Minimum Inhibitory Concentration (MIC) of bacteria by the dilution method on Mueller Hinton Broth (MHB) in culture tubes and Minimum Bactericidal Concentration (MBC) of bacteria Aeromonas hydrophila by garlic (Allium sativum) and Zataria multiflora hydro alchoholic extracts on Mueller Hinton Agar (MHA) plates were determined. Based on the results MIC of bacteria Aeromonas hydrophila of ethanol extracts of Zataria multiflora and garlic, respectively, 0.25 and 1 mg/ml and MBC of ethanol extracts of Zataria multiflora and garlic respectively 0.5 and 2 mg/ml was determined and found Zataria multiflora extract has strong antibacterial effects of garlic extract.
معاونت پژوهش و فناوری دانشگاه آزاد اسلامی واحد لاهیجان
Journal of Aquaculture Development
2322-3545
10
4
2017
1
1
Relationship between white blood cells and gill tissue lesions in Flower horn fishes fed with cow skin hydrolyze
133
142
FA
A.
Vosoughi
Department of Marine Biology, North Tehran Branch, Islamic Azad University, Tehran
Sh.
Dadgar
Iranian Fisheries Science Research Institute (IFSRI) of Research, Education and Extension Organization (AREEO), I.R. of IRAN
A.
Veisi
Department of Marine Biology, North Tehran Branch, Islamic Azad University, Tehran
In This study that was accomplished to investigate the relationship between levels of white blood cells (WBC) and gill tissue lesions in Flower horn, at first the number of 216 Flower horn fishes with average weight of (0/95 0.2) g and average length of (3.7 0.2) mm in 6 treatments, each treatment with three replication for 100 days with diets containing different levels of cow skin hydrolyze were fed. Treatment (T1) containing 20%, (T2) containing 40%, (T3) containing 60%, (T4) containing 80%, (T5) containing 100% replacement of cow skin hydrolyze instead of fish meal and control diet (T6) had no cow skin hydrolyze. After 100 days the gill samples of each treatments, was excised randomly (each treatment 9 gill speciments) and obtained samples were bleeded. then from gill of that bleeded fishes, tissue samples were prepared. The results of comparison between the levels of WBC of these fishes and assessment of gill tissue samples showed that in the treatments (T4) and (T5) that had more hydrolyzed powder in its diets, in comparison to other treatments, along with an overall reduction in the level of WBC and percent of lymphocytes, had more lesions in its gills.