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:: Volume 15, Issue 1 (3-2021) ::
JAD 2021, 15(1): 1-18 Back to browse issues page
Optimization of UV radiation method for DNA degradation of Siberian sturgeon (Acipenser baerii) spermatozoa in gynogenesis studies: Comet assay
Mohammad Hassanzadeh Saber * , Hossein Zolgharnein , MohammadAli Salari Aliabadi , MohammadAli Yazdani Sadati , Mahtab Yarmohammadi
Department of Genetics & Biotechnology, International Sturgeon Research Institute, Agricultural Research, Education and Extension Organization, Rasht, Iran
Abstract:   (1108 Views)
Induction of gynogenesis requires the spermatozoa with degraded DNA with high motility, simultaneously. In this study, to obtain this capability in Siberian sturgeon spermatozoa, UV radiation with a dose of 4257 j/m2 was applied. First, the seminal fluid was separated from the spermatozoa using a centrifuge, and then 10% of the sperm was prepared and irradiated with UV radiation for 30, 60, 90, 120, and 150 seconds to obtain the best motility and percentage of DNA damage using the comet assay method in comparison with the control. Simultaneously, the fertilization ability of these sperms was tested in sterlet sturgeon eggs and the rate of hatched larvae. Based on the results, the best duration of irradiation of spermatozoa was determined to as 90 seconds. The DNA degradation rate was 32.27±1.96 percent, the motility rate was 50% with forwarding movement, the fertilization rate was 52.1±0.52 percent, and the egg hatching rate was 1.1±0.11 percent. Therefore, genetically inactivated Siberian sturgeon sperm could be used in interspecific gynogenesis studies in sturgeon. Either to producing offspring of all females or to protect their extinction.
Keywords: Siberian sturgeon, spermatozoa, comet assay, fertilization, hatching.
Full-Text [PDF 907 kb]   (331 Downloads)    
Type of Study: Research | Subject: Special
Received: 2021/01/4 | Accepted: 2021/02/28 | Published: 2021/03/30
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Hassanzadeh Saber M, Zolgharnein H, Salari Aliabadi M, Yazdani Sadati M, Yarmohammadi M. Optimization of UV radiation method for DNA degradation of Siberian sturgeon (Acipenser baerii) spermatozoa in gynogenesis studies: Comet assay. JAD 2021; 15 (1) :1-18
URL: http://aqudev.liau.ac.ir/article-1-736-en.html


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Volume 15, Issue 1 (3-2021) Back to browse issues page
نشریه توسعه آبزی پروری Journal of Aquaculture Development
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