The effect of different dietary zinc on Pyruvate Kinase activity and electrophoretic pattern of muscle in Caspian Kutum
(Rutilus frisii kutum, Kamensky, 1901)
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Katayoun Karimzadeh * , Askar Zahmatkesh |
Department of Marine Biology, Lahijan Branch, Islamic Azad University, Lahijan, Iran |
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Abstract: (2558 Views) |
A feeding trial with two zinc levels (50 and 200 mg/kg) and control level (0 mg/kg of zinc) were conducted to investigate the pyruvate kinase (PK) activity and electrophoretic pattern of muscle in Caspian Kutum (Rutilus frisii kutum). Two hundred and seventy juvenile fish (0.56±0.06g; mean ±SD) were randomly distributed in 9 aquaria (50L) and were fed three times daily for 3 months. Muscle protein expression, pyruvate kinase activity and zinc concentration were detected in Caspian Kutum muscle. Results showed that the highest amount of zinc was observed in fish fed 200mg/kg zinc in diet. Electrophoretic pattern of proteins extracted from the muscle were mostly constituted by myosin heavy (HC) and light (LC) chains which are characterized with three different protein regions ( LC1F, LC2F, LC3F). During the experimental period changes were found in LC2F and LC3F amounts. Pyruvate kinase activity showed a significant increase at the end of the experiment. Significantly positive correlation was observed between weight gain and pyruvate kinase activity in fish fed 50 mg/kg zinc (r=0.95; p≤0.05) .In conclusion, it appears that dietary zinc with 200 mg/kg did not affect intensity of separated protein bands and PK activity of muscle in Caspian Kutum. |
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Keywords: Rutilus frisii Kutum, Zinc, Pyruvate kinase, Protein Pattern |
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Full-Text [PDF 1097 kb]
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Type of Study: Research |
Subject:
Special Received: 2016/03/6 | Accepted: 2016/07/12 | Published: 2017/11/27
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