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Evaluation of three algal strains isolated from Miankaleh wetland for biodiesel production and quantitative study of three genes expression from Chaetomorpha antennina as response to surplus light and CO2 supply
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Mehrdad Asadian   , Barat Ali Fakheri , Ahmad Farhad Talebi * , Nafiseh Mahdinezhad , Shahrokh Gharanjik |
| Department of Microbial Biotechnology, Faculty of New Sciences and Technologies, Semnan University, Semnan, Iran |
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Abstract: (1426 Views) |
Algae by consuming CO2, light, and nutrients in wastewater not only could reduce the environmental pollutants, but also could increase the productivity of biomass and value-added byproducts such as biofuels in their cells. On the other hand, molecular techniques such as Real-Time quantitative PCR (qPCR) helps to better identify the pathways of response to environmental conditions in algal strains. In this study, at the first, the properties of prospective biodiesel were evaluated for three algal strains (Chaetomorpha antennina, Ulva intestinalis, and Bryopsis pennata) grown on different light intensity (8100, 12150, and 16200 lux) and different CO2 concentrations (380, 5190, and 10000 ppm). Finally, the expression profile of three genes involved in CO2 sequestration (CA, CIA5) and light harvesting (CAB) was investigated in superior strain. The results showed that the light intensity of 12150 lux and CO2 concentration of 5190 ppm led to the highest lipid content (30 to 37%) for all three algal strains. Also, all microalgae used in this study have a good ability to produce biofuels. Moreover, it was observed that increasing CO2 concentration has led to a decrease in the expression of the CA gene, but the highest expression of the CIA5 gene was observed in 5190 ppm CO2 concentration. The highest CAB gene expression was also observed in 12150 lux. In summary, the control of expression of the involved genes could significantly determine the final lipid productivity as well as the properties of prospective biodiesel. |
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| Keywords: Biodiesel, Gene Expression, CO2 sequestration, Light intensity. |
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Full-Text [PDF 865 kb]
(398 Downloads)
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Type of Study: Research |
Subject:
Special
Received: 2020/01/31 | Accepted: 2020/06/14 | Published: 2021/12/31
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