|
Effect of Lactobacillus plantarum and Bacillus subtilis bacteria on growth and hematological indices of rainbow trout (Oncorhynchus mykiss) fry immunized with bivalent streptococcosis/lactococcosis vaccine and yersiniosis vaccine
|
Masomeh Darvishi1   , Farid Firouzbakhsh * , Sakineh Yeganeh3 , Roghayeh Safari4 |
1- M_darvishi_m71@yahoo.com
Fisheries group, Faculty of Animal Science and Fisheries, Sari University of Agricultural Sciences and Natural Resources, Sari, Iran., f.firouzbakhsh@yahoo.com , f.firouzbakhsh@sanru.ac.ir
3- skyeganeh@gmail.com
4- fisheriessafari@yahoo.com |
|
|
Abstract: (180 Views) |
Introduction: The development of intensive aquaculture has led to the emergence of various bacterial diseases, the outbreak of which in fish farms may be caused by single or multiple pathogens. Considering that infectious diseases are known as a big obstacle in the direction of aquaculture and its supply to the world market, for the prevention and control of disease, veterinary drugs and recently vaccination are mainly used in aquaculture farms.
Material and Methods: This research was conducted to investigate the effect of Lactobacillus plantarum and Bacillus subtilis bacteria as probiotics, both alone and in conjunction with a bivalent vaccine for streptococcosis/lactococcosis and a yersiniosis vaccine in rainbow trout fry with an average weight of 3.35 ± 0.3 grams, across 4 treatments and 3 repetitions over a period of 60 days. The experimental groups included the first treatment (control) consisting of fish fed a basal diet without vaccine administration, the second treatment with fish fed a diet containing 1 gram of probiotic per kilogram of feed without vaccine administration, the third treatment with fish fed a basal diet along with vaccine administration, and the fourth treatment with fish fed a diet containing 1 gram of probiotic per kilogram of feed along with vaccine administration.
Result and Discusion: The results showed that the growth indices and survival rates of the fed fish did not have a significant difference compared to the control group (P>0.05). In the results of the samples taken on day 30, no significant differences were observed in the factors of RBC, Hb, HCT, MCV, MCH, and MCHC compared to the control group (P>0.05). In the results of the samples taken on day 60, the number of red blood cells (RBC) in treatments 3 and 4 was lower than that of the control group (P<0.05), while the MCV levels in groups 3 and 4 on day 60 showed an increase compared to the control group (P<0.05). On day 30, the percentage of lymphocytes in the vaccinated groups showed a significant increase compared to the control group (P<0.05), while no significant changes were observed in the percentage of neutrophils, monocytes, and eosinophils at 30 and 60 minutes (P>0.05).
Conclution; Based on the results obtained from the present study, it can be concluded that the administration of 1 gram of the specified probiotic in fish vaccinated with two types of bivalent vaccines for streptococcosis/lactococcosis and yersiniosis did not have a significant positive effect on growth and hematological indices. |
|
| Keywords: Lactobacillus plantarum, Bacillus subtilis, Growth, hematological, vaccine. |
|
|
|
Type of Study: Research |
Subject:
Special
Received: 2024/03/29 | Accepted: 2024/04/11 | Published: 2025/07/1
|
|
|
|
|
|
|
| Send email to the article author |
|
|
|
| Add your comments about this article |
|
|
|
