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:: Volume 9, Issue 4 (1-2016) ::
JAD 2016, 9(4): 43-51 Back to browse issues page
Polymorphism investigation of SLC24a5 gene in rainbow trout (Oncorhynchus mykiss)
Mehrdad Karimkhani , Siyamak Yousefi Siahkalroodi* * , Ghobad Asgari Jafarabadi
Abstract:   (2635 Views)

In this survey polymorphism of SLC24a5 gene of Rainbow trout (Oncorhynchus mykiss) was studied. Samples were prepared from 100 Rainbow trout muscles. After DNA extraction, part of gene from polymorphism area with 1000 bp amplified by PCR then cut with Taq1 inhibiting enzymeand electrophoresed on agarose gel 1.5%. Results shows that M and N alleles are in this area of gene, and the frequency of them is 0.72 and 0.28 in population respectively. MM and NN genotypes were distinguished which frequency of them were estimated 0.72 and 0.28 in whole population respectively. Chi-Square test for this part of SLC24a5 gene was not similar with Hardy–Weinberg equilibrium. It shows non-random mating, small population and increase in inbreeding and homozygotes increase in the population of this study. Furthermore Rainbow trout is so important in aquaculture industry in Iran the notability of this issue is significant because SLC24a5 gene is highly inheritable and selection of that can improve the color of meat.

Keywords: polymorphism, Rainbow trout (Oncorhynchus mykiss), slc24a5, PCR-RFLP
Full-Text [PDF 843 kb]   (1217 Downloads)    
Type of Study: Research | Subject: Special
Received: 2016/04/28 | Accepted: 2016/04/28 | Published: 2016/04/28
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Karimkhani M, Yousefi Siahkalroodi* S, Asgari Jafarabadi G. Polymorphism investigation of SLC24a5 gene in rainbow trout (Oncorhynchus mykiss). JAD 2016; 9 (4) :43-51
URL: http://aqudev.liau.ac.ir/article-1-313-en.html


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Volume 9, Issue 4 (1-2016) Back to browse issues page
نشریه توسعه آبزی پروری Journal of Aquaculture Development
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