Abstract: To evaluate the effects of using Artemia fransiscana enriched with cod liver oil and vitamin E on growth, survival and salinity and thermal stress resistance of Sepia pharaonis larvae this study was conducted. 25 days after hatching the larvae of Sepia pharaonis larvae with average length of 15.97±0.15 mm, 3.53±0.03 gr body weight transferred into culture tanks in five treatments (each with 3 replicates). Experimental treatments include: Artemia fransiscana enriched with cod liver oil at different levels of E1= 5%, E2= 10% and E3=15 % vitamin E , non-vitamin cod liver oil, and control (non-enriched artemia) group. . In each replicate, the 20 larvae were introduced into tanks of 20 liters and 10 artemia /liter/ day to feed larvae were used. At the end of experiment, To investigate stress resistance, at the end of experiment three groups of larvae were encountered at one hour stress with 5, 15, 25 ppt salinity , and 10, 25 ,32 Ċ temperature levels. The results showed that feeding with vitamin E elevate survival and growth factors of Sepia pharaonis larva, But did not show a significant difference with control (P > 0.05), In the event that HUFA treatment showed a significant difference with other groups Statistically(P < 0.05). The results of this research showed that unsaturated fatty acids can improve growth and survival in cuttlefish larvae, but vitamin E did not exhibit any effect on survival rate, growth and stress resistance (P >0.05).