One of the ways to increase immunity and health level of fis hes is to apply diet supplements such as probiotics. This project carried out with the aim of Enterococcus species and its usage on feeding, also its effects on blood & immunity factors of sturgeon fingerlings in International Sturgeon Research Institute during 2010-2014. A total of 96 sturgeon fingerlings with mean weight of 9.24±0.14g and mean total length of 14.39±0.4cm and density of 8 pieces were stocked in 12 fiberglass vans with 500L volume having 1m height and diameter, the water supplied commonly by SephidRud River and well with aerator. The treatments were completely random (4 treatments, with 3 replications per treatment). In treatments one, two and three, the fingerlings consumed the diet supplemented with Enterococcus faecalis as 107, 108 and 109 CFU (in per gram diet), respectively. No, Enterococcus faecalis added to diet in control group. In an in vivo work the effect of Enterococcus faecalis as the supplementary diet was evaluated on growth performance of Persian sturgeon for a period of 60 days. Strong growth of all LAB were observed at 20˚C and 30˚C, as well as 4%, and 8% (w/v) NaCl. Use of diet supplemented with Enterococcus faecalis (JF831161) resulted in a significant improvement of lysozyme activity, IGM, hemoglobin, white and red blood cells, hemoglobin and neutrophils. These results showed that use of Enterococcus faecalis can act as a positive probiotic in Persian sturgeon feed via improvement of fish immunity and health.