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Efficacy of Injectable monovalent and bivalent Vaccines against Streptococcosis and yersiniosis in rainbow trout
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Sseyed Abdolhamid Hosseini *   , Mojtaba Alishahi2 , Abolhassan Rastiannasab2 , Roghayeh Mahmoudi2 , Habibola Gandomkar2 |
Shahid Motahary Cold water Fishes Genetic and breeding Research Center, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Yasoj, Iran, -
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Abstract: (1031 Views) |
In this study, the efficacy of Streptococcus and Yersinios vaccine in form of bivalent and monovalent was compared in rainbow trout (Oncorhynchus mykiss). Three hundred fishes (25±2g) were randomly divided into four equal groups in triplicates. Treatments contains Streptococcosis vaccine, Yersiniosis vaccine, bivalent vaccine and control group. Fish in vaccinated treatment intraperitoneal injected with 100 microliter vaccine on the first day. Blood samples were collected from vaccinated and non-vaccinated groups at days 0, 30, and 60 of study and antibody titer were subsequently performed. Sixty days after initial immunization all groups were challenged by lethal dose of Streptococcus iniae and Yersinia rockeri, mortality rate was calculated and compared between the groups for 14 days.
The results demonstrated that lysozyme rate in bivalent vaccine were significantly higher at day 60 post-immunization compared to the control group. A significant increase was observed in the complement activation in bivalent vaccine at day 30 and 60 post-vaccination compared to the other groups(p<0.05). Number of WBC in vaccinated groups were higher than control treatment.
High antibody levels were observed in the vaccinated fish at day 30 and 60 post-immunization using both vaccines(p<0.05). The percentage of trout cumulative mortality after the challenge with Streptococcus iniae and Yersinia rockeri showed a significant decrease in vaccinated groups. Generally, it can be concluded that the administration of bivalent Streptococosis/Yersiniosis vaccine to the rainbow trout provided appropriate protection against disease and effectively induced the immune response against these two diseases which is comparable to either of the vaccines separately.
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| Keywords: Bivalent and Monovalent vaccine, Streptococosis, Yersiniosis |
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Full-Text [PDF 581 kb]
(431 Downloads)
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Type of Study: Applicable |
Subject:
Special
Received: 2021/01/16 | Accepted: 2021/03/6 | Published: 2023/05/31
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