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:: Volume 11, Issue 1 (4-2017) ::
3 2017, 11(1): 1-7 Back to browse issues page
Polymorphism Investigation of Tyrosinase Gene in Golden Rainbow Trout (Oncorhynchus mykiss)
B. Akbarnejad , S. Yousefi Siahkalroodi , Sh. Zare Karizi
Department of Animal Sciences, Faculty of Agriculture, Varamin-Pishva Branch, Islamic Azad University, Varamin
Abstract:   (1009 Views)

In order to identify polymorphisms in the Tyrosinase gene for Golden rainbow trout (Oncorhynchus mykiss), a total of 100 different samples (fry) were prepared and DNA extraction was performed from their caudal fins. The polymerase chain reaction (PCR) was amplified fragment of 423bp the Tyrosinase gene. The amplified fragments were using specific primers (Tyr1.2r and Tyr1.2f) and Hinf1 cutting enzyme. In enzymatic digestion of the PCR products was observed three restricted patterns in 222bp and 201bp with NN (26 samples), and 423bp with MM (36 samples). The number of alleles N and M were 90 and 110 respectively, the gene frequency for allele N and M were .45 and .55 respectively. The number of genotypic frequencies NN, MN and MM were .26, .38 and .36 respectively. The results showed that deviations from Hardy -Weinberg equilibrium were in all cases, due to artificial reproduction, probably.

Keywords: Polymorphism, Golden Rainbow trout (Oncorhynchus mykiss), Tyrosinase, PCR-RFLP
Full-Text [PDF 843 kb]   (345 Downloads)    
Type of Study: Research | Subject: Special
Received: 2017/04/13 | Accepted: 2017/05/1 | Published: 2017/06/24
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Akbarnejad B, Yousefi Siahkalroodi S, Zare Karizi S. Polymorphism Investigation of Tyrosinase Gene in Golden Rainbow Trout (Oncorhynchus mykiss). 3. 2017; 11 (1) :1-7
URL: http://aqudev.liau.ac.ir/article-1-457-en.html

Volume 11, Issue 1 (4-2017) Back to browse issues page
نشریه توسعه آبزی پروری Journal of Aquaculture Development
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