[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Articles archive::
For Authors::
For Reviewers::
Registration::
Contact us::
Site Facilities::
::
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
:: Volume 12, Issue 3 (5-2018) ::
JAD 2018, 12(3): 91-103 Back to browse issues page
The introduction of serum proteins, purification and molecular weight determination of immunoglobulin (IgM) in Oncorhynchus mykiss and Ctenopharyngodon idella
Ahmad GHOROGHI * , Alireza Rezvani Gilkolahi
Iranian Research and Fisheries Science Institute
Abstract:   (2389 Views)
      In this research paper quantities and properties of proteins and immunoglobulin’s of the Oncorhynchus mykiss and Ctenopharyngodon idella in normal physiological conditions, weather of Iran was studied. Total quantities of immunoglobulin (IG) in mammals, such as mice and humans, typically between 15-20 mg per mL and about 25% of the proteins that make up blood serum. This amount, immunoglobulins, IgM 1-2 mg per ml or 5/1 rate (one and a half) percent of the total concentration of the serum proteins. In this study it was found that while the bony fishes, but not mammalian diversity immunoglobulin IgM concentration in the blood serum protein in farmed salmon and grass higher than immunoglobulin (IG) mammals. It was also found in serum total protein concentration of salmon and grass breeding in our country is generally lower than the total protein in mammalian blood serum. Review of the studies and research by others as well as our own measurements show that there is no clear answer to this difference. Isolation and purification of serum immunoglobulin (IG) fish was found via using fish serum antibody production in rabbits and purification of antibody by dialysis and analyzed using electrophoresis and affinity chromatography, Molecular weight of heavy chain immunoglobulin was 82 kDalton and light chains was about 21 kDalton in Oncorhynchus mykiss and the heavy and light chains immunoglobulin in Ctenopharyngodon idella were determined 70.4 and 25.3 kDalton respectively.
Keywords: serum proteins, purification, molecular weight, immunoglobulin (IgM), Oncorhynchus mykiss, Ctenopharyngodon idella
Full-Text [PDF 736 kb]   (2443 Downloads)    
Type of Study: Applicable | Subject: Special
Received: 2015/08/29 | Accepted: 2016/12/5 | Published: 2019/01/6
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

GHOROGHI A, Rezvani Gilkolahi A. The introduction of serum proteins, purification and molecular weight determination of immunoglobulin (IgM) in Oncorhynchus mykiss and Ctenopharyngodon idella. JAD 2018; 12 (3) :91-103
URL: http://aqudev.liau.ac.ir/article-1-193-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 12, Issue 3 (5-2018) Back to browse issues page
نشریه توسعه آبزی پروری Journal of Aquaculture Development
Persian site map - English site map - Created in 0.05 seconds with 36 queries by YEKTAWEB 4645